In order to find an effective improvement method for acid mango-planting soil，a field experiment was conducted to study the effects of lime，organic fertilizer，lime combined with organic fertilizer on the biological and chemical properties of acid soil and mango growth and quality.The results indicated that only organic fertilizer or only lime significantly increased soil cation exchange capacity and base-exchangeable ions.Only organic fertilizer significantly improved the contents of organic matter，alkali-hydrolyzable N and available P，but had no significant effect on soil acidity.Only lime significantly reduced soil acidity，but had no significant effect on soil nutrients.Under combined application of lime with organic fertilizer，hydrolyzed acid and exchangeable acid reduced，soil pH increased by 0.38 units，the content of organic matter，alkali-hydrolyzable N and available P was reduced by 62.01%～78.38%，soil cation exchange capacity and base-exchangeable ions increased，and base saturation rose by 13.37 percentage points.Only lime and combined with organic fertilizer increased the activities of soil urease，invertase and catalase by 7.63%～54.20%，and decreased the activity of soil acid phosphatase by 12.34%～27.06%，while all the four enzymes’ activities increased by 4.55%～58.75% for only organic fertilizer treatment.Single or combined application of lime and organic fertilizer improved soil microbial activity，chlorophyll content，specific leaf weight，fruit weight，fruit quality and yield.The leaf quality，fruit yield and fruit soluble solids of the single application of organic fertilizer were higher than that of the single application of lime，but the sugar acid ratio of fruit was lower than that of the single application of lime.In a word，the effect of combined application was the best，whose soil microbial biomass carbon，soil microbial quotient，fruit weight，sugar-acid ratio and yield increased by 163.85%，48.73%，20.88%，64.00%，54.70%，respectively.In conclusion，only organic fertilizer or only lime could improve the acid soil of mango garden to a certain extent，but the effect of mixed application of lime and organic fertilizer was the best.Therefore，combined application of lime with organic fertilizer is an effective and sustainable method for improving acid soil of mango plantation.