Aiming at the problem of secondary salinization soil in vegetable field，the effects of different dosages of soil conditioner and its combined application with microbial fertilizer on promoting soil desalination，improving soil physical and chemical properties and soil microbial diversity were studied.The results showed that after applying soil conditioner and microbial fertilizer，soil physical and chemical properties were significantly improved，soil bulk density，EC，total salt content all decreased to varying degrees，Na+，K+，Ca2+，Cl-，HCO3- content decreased，and soil salt return was effectively inhibited.Moreover，after applying soil conditioner and microbial fertilizer，the diversity and richness of soil bacterial community tended to increase，especially the number of nitrogen-fixing bacteria and bacteria that decompose organic matter significantly increased.The increase of soil beneficial bacteria content promoted the growth of cucumber.Soil conditioner and microbial fertilizer had significant effects on improving soil physical and chemical properties，reducing soil surface salinity，and increasing soil microbial and bacterial community diversity，which could provide reference for alleviating salt stress in secondary salinization soil of vegetable field.