In this study， the soil phosphorus fractions were classified by continuous extraction method for soils collected from the livestock farm wastewater irrigation and conventional fresh water irrigation farmlands in different soil depths.The content of each phosphorus form in different soil layers under livestock wastewater and fresh water irrigation was analyzed and compared. The distribution and transformation characteristics of soil phosphorus form were analyzed.Results showed that the contents of water-soluble-Pi and NaHCO3-Pi in each soil layer after irrigation with livestock wastewater significantly increased compared with those with fresh water， and the proportion of the sum of water-soluble-Pi and NaHCO3-Pi (active phosphorus) in the soil layer of 0～20 cm increased by 15.03 percentage points.Livestock wastewater irrigation also significantly increased the content of NaOH-Pi in 0～20 and 20～40 cm soil layers， but the increase of the active phosphorus content in each soil layer was significantly higher than that of NaOH-Pi， indicating that livestock wastewater irrigation significantly impacted the active phosphorus.At the same time， the proportion of HCl-Pi， organic phosphorus， and residual-P in total phosphorus in each soil layer decreased， thus reducing the fixation of phosphorus.Results all showed that the content of soil available phosphorus form increased after livestock wastewater irrigation， which was of great significance for the growth and development of wheat.