The responses of organic acids and amino acids secreted by roots during the vegetating period of rape to different fertilization treatments were explored to provide a theoretical basis for safe and efficient production of rape.The dry weight of roots and above-ground parts in rape and the types and contents of organic acids and amino acids secreted by the roots were analyzed in pot experiment with five different proportioned fertilizers.The results showed that malonic acid， acetic acid and citric acid were the main organic acids secreted by the roots of rape， among which malonic acid and acetic acid were the most significantly affected by fertilization， and organic fertilizer combined application significantly reduced their secretion.Compared with single application of chemical fertilizer， combined application of organic fertilizer significantly reduced the total amount of organic acids secreted by the roots of rape， and reduced the biomass of above-ground and root in different degrees.When the proportion of chemical fertilizer and organic fertilizer application was 1∶3， the total organic acid decreased most obviously， but there was no significant difference in biomass compared with single chemical fertilizer application.Glutamic acid and γ-amin butyric acid were the main amino acids secreted by the roots of rape.Different ratios of combined application had little effect on the 35 amino acids species secreted by the rape roots， but had significant effect on the total amino acid secretion.With the increase of organic fertilizer ratio， the total amount of amino acids showed a trend of decreasing first and then increasing.The dry weight of the root was extremely significantly positively correlated with the total amount of organic acid secretion， but not with the total amount of amino acid secretion， which was significantly correlated with the content of soluble organic carbon and the number of bacteria in the soil.We speculated that the ecological effects of organic acids and amino acids secreted by the root of rape may be different， the former may be the stress response of plants under nutrient stress， while the latter may be to enhance soil activity.The suitable ratio of chemical fertilizer to organic fertilizer is 1∶3， which can provide the most balanced nutrition for the growth of rape and provide reference for the fertilization management of rape.