Garlic Root Rot is one of the main diseases of garlic in Jinxiang county and its surrounding areas of Shandong province.In this study， 62 strains of potential pathogenic fungi of garlic Root Rot were isolated and purified from 51 samples of garlic Root Rot collected for 3 consecutive years by conventional tissue isolation method.Morphological and molecular biological identification showed that the main groups of these fungi were Fusarium and Setophoma.According to Koch’srule， the Fusarium H9 was confirmed to be the pathogen of garlic root rot.The pathogenicity of garlic root rot pathogen H9 with different concentrations on garlic seedlings was studied by hydroponics experiment in greenhouse.The results showed that the pathogenicity was positively corelated with the inoculation amount.Serious Root Rot appeared in garlic seedlings after 40 days inoculation with spore concentration of 2×109 cfu/mL， inoculation amount of 100 and 150 mL， respectively.In summary， the main pathogenic fungi of Root Rot of garlic in Shandong and its surrounding areas are Fusarium and Setophoma， which provide a theoretical basis for the scientific control of garlic Root Rot in this area.