Under long-term rice straw return condition，fertilizer management methods which can increase rice yield and fertilizer use efficiency have been integrated to see if they can increase rice yield and rice nitrogen (N) uptake in red paddy soil.Four-year (2013～2016) experiment was carried out in Dufu farm in Gaoan county，Yichun city，Jiangxi province.Six fertilizer management methods were chosen，including partial substitution of fertilizer with manure and/or slow release fertilizer，incorporation of microelement (Si，Zn，and S)，and nitrogen input decreased by 20%.Rice yield and straw weight were measured at each season.Nitrogen and phosphate uptake in rice and straw were measured.Both nitrogen use efficiency and agronomic efficiency of fertilizer N were calculated.Soil properties and microbial biomass were analyzed after the late rice was harvested in 2016.The results showed that there were no significant differences in rice yields under different fertilizer treatments.Above ground N uptakes were significantly higher under manure application (P<0.05).Rice yield decreased when fertilizer N was cut by 20%；however，the decrease was not significant.Above ground N uptake decreased significantly under 20% N cutoff.Early rice yield and late rice yield varied in different years.Late rice yield was not always larger than early rice yield.Nitrogen agronomic efficiency for early and late rice was 20.6～24.8 kg/kg and 14.5～18.4 kg/kg，respectively.Nitrogen recovery fraction for early and late rice was 31.6%～40.0% and 26.0%～35.4%，respectively.Nitrogen recovery fraction for slow release fertilizer was larger than other fertilizer treatments，but the difference was not significant (P=0.07).There were no significant differences in soil organic matter，total N，pH，and microbial biomass C，microbial biomass N in arable layer under all treatments except available potassium and phosphate.Double rice yield，soil organic matter and total N did not increase under manure incorporation，micronutrient input and slow release fertilizer application over four-year field experiment.This implied that long-term straw return had great effects on rice yield，therefore，it takes longer time to increase rice yield to a significant level under manure incorporation.Furthermore，soil provided enough micronutrients for double rice growth under straw and manure application because extra application of micronutrients did not result in an increase of rice yield and N uptake over eight seasons.