The chemical fertilizers combined with organic fertilizers is one of main technical measures to achieve the fertilizer reduction.The effects of chemical fertilizer combined with organic fertilizer on the rapeseed yields，the nutrient accumulation and the nitrogen fertilizer efficiency were studied in rapeseed production along the Yangtze River regions in Anhui province.A split field experiment was conducted and the main treatment was orgamic forticizor application level， composed of two levels including with chemical fertilizer with no organic fertilizer and chemical fertilizer with 2250 kg/hm² organic fertilizer.The sub-treatment was the nitrogen application level，including 0，90，135，180，225，270 kg/hm² treatments.The rapeseed yields，the nutrient accumulation and the nitrogen use efficiency were analyzed.The rapeseed yields achieved stable level at more than 135 kg/hm² of the application of nitrogen.The stable yield (3005 kg/hm²) at only chemical fertilizer applied was attained when the optimum amount of nitrogen fertilizer was 141 kg/hm² fitting by the linear plus plateau model.This yield was closer to that of the treatment (3277 kg/hm²) with 90 kg/hm² nitrogen fertilizer combined with 2250 kg/hm² organic fertilizer and the application of nitrogen fertilizer was reduced by 36.17%.The rapeseed yields with nitrogen fertilizer combined with organic fertilizer were increased by 11.29% ～ 134.57% than that with only chemical fertilizer applied at the same amount of nitrogen fertilizer.The highest rapeseed yield was reached at the 180 kg/hm² nitrogen fertilizer combined with 2250 kg/hm² organic fertilizer，and the increase rate of rapeseed was decreased with the increase of nitrogen fertilizer compared with the treatments with only chemical fertilizer yet.There was significant correlation between the rapeseed yield and the pod number per plant with the chemical fertilizer only，and the yield of rapeseed with organic fertilizer was significantly correlated with the pod number per plant and the seed per pod.The nutrient accumulation of nitrogen，phosphorus and potassium of treatments with chemical fertilizer combined with organic fertilizer were higher than those of treatments with chemical fertilizer applied only.When the amount of nitrogen fertilizer applied was the same，the increase rate of the nutrient accumulation with the organic fertilizer participation was reduced by the increase of nitrogen fertilizer compared with that of treatments with chemical fertilizer applied.All the nitrogen fertilizer efficiencies were improved by the application of the organic fertilizer.The nitrogen partial factor productivity and nitrogen agronomic efficiency of the treatment with 90 kg/hm² nitrogen fertilizer combined with 2250 kg/hm² organic fertilizer were higher than that of the optimum amount of nitrogen fertilizer without organic fertilizer applied by 68.18% and 74.76%，respectively.The optimum amount of nitrogen fertilizer was 141 kg/hm² with stable rapeseed yield (3005 kg/hm²) attained when only chemical fertilizer applied.The highest rapeseed yield was achieved at the application of the nitrogen fertilizer 180 kg/hm² combined with 2250 kg/hm² organic fertilizer.The application of organic fertilizer increased the rapeseed yield by increasing the pod number per plant and the seed per pod.The nutrient accumulation and nitrogen fertilizer efficiency were both increased with the addition of organic fertilizer.The addition of organic fertilizer did not only reduce the amount of nitrogen fertilizer applied by 36.17%，but also increased the nitrogen fertilizer efficiency.