To explore the effects of zinc(Zn)，molybdenum(Mo)，nitrogen(N)，phosphorus(P)，potassium(K) and organic fertilizers on the yield of Salvia miltiorrhiza and the content of salvianolic acid and tanshinone，and to find out the best organic and inorganic fertilizer combination scheme suitable for the cultivation of Salvia miltiorrhiza in Zhongjiang county，Siclom prvince，four factors and five levels quadratic orthogonal rotation combination design was adopted to establish the function model of each factor and objective function.Based on the analysis of single factor effect and interaction effect，frequency optimization method was applied to obtain the fertilization scheme of Salvia miltiorrhiza with high quality and high yield.Low Zn，low NPK and high Mo were beneficial to the yield improvement，but organic fertilizer had no significant effect on the yield of Salvia miltiorrhiza.Low NPK and high organic fertilizer were beneficial to the accumulation of salvianolic acid，while Zn and Mo fertilizers had no significant effect on the accumulation of salvianolic acid.Low Zn，low Mo，high NPK and high organic fertilizer were beneficial to tanshinone accumulation.The effects of different fertilizer interactions on yield，salvianolic acid and tanshinone content were different.In the confidence interval，the yield of Salvia miltiorrhiza，the content of salvianolic acid and the content of tanshinone reached the highest combination，and the optimal application scheme of organic and inorganic fertilizer with high quality and high yield was obtained.Rational combination of Zn，Mo，NPK with organic fertilizer can improve the yield of Salvia miltiorrhiza and the content of salvianolic acid and tanshinone.The optimal combination range is:Zn fertilizer 41.96～48.04 kg/hm²；Mo fertilizer 0.84～0.96 kg/hm²；N fertilizer 210.49～239.51 kg/hm²；P fertilizer 111.26～127.74 kg/hm²；K fertilizer 140.33～159.68 kg/hm²；organic fertilizer 1755.90～1844.10 kg/hm².