In order to improve the application method of nitrogen fertilizer for middle rice in Jianghan Plain，a field trial was conducted and 4 local nitrogen fertilizer managements including conventional fertilization (FFP)，slow-controlled release fertilizer and urea combined application (CRF)，seaweed polysaccharide nitrogen fertilizer replacement (HTN) and no nitrogen control (CK) were set up to study the effects of different nitrogen fertilizer management on the nitrogen absorption，transportation and nitrogen utilization efficiency of machine transplanted rice and direct seeded rice.The result showed that:in early tillering of rice，the dry matter accumulation of machine transplanted rice with FFP treatment was 41.83% and 19.89% higher than those of CRF and HTN treatment，respectively；dry matter accumulation of direct seeded rice with CRF treatment was 4.84% higher than FFP.The ratio of dry matter accumulation and nitrogen accumulation to the total dry matter accumulation and nitrogen accumulation of machine-transplanted rice at tillering stage-joining stage was 12.58%～17.21% and 15.24%～67.24% higher than that of direct seeding rice，respectively.The dry matter accumulation of each treatment at the jointing stage to maturity stage was expressed as CRF>HTN>FFP，and the proportion of dry matter accumulation of direct-seeded rice to total dry matter accumulation was 12.72%～17.61% higher than that of machine-transplanted rice.Compared with FFP， the spike dry matter accumulation of machine transplanted rice and direct seeded rice in CRF treatment increased by 19.71% and 14.82% respectively， and that in HTN treatment increased by 13.10% and 3.93% respectively. In terms of nitrogen accumulation， transport and utilization， machine transplanted rice is similar to seeded seeding rice， which is CRF > HTN > FFP.Compared with FFP，the apparent nitrogen utilization efficiency of machine transplanted rice and direct seeding rice under CRF treatment increased by 38.02% and 34.57%，respectively，and HTN treatment increased by 11.82% and 13.33%，respectively.The combined application of slow-release nitrogen and urea and the application of seaweed polysaccharide nitrogen fertilizer are all conducive to increasing the accumulation，transportation and utilization of nitrogen in hybrid mid-season rice.At the same time，proper backward shift of nitrogen fertilizer helps direct-seeded rice nitrogen absorption.