Rhizospheric soil of loquat was irrigated with Trichoderma P3.9 spore suspension. Fungal diversity and community in rhizospheric soil were tested by high throughput sequencing of Illumina platform. The results showed that the dominant fungi were Fusarium，Fusarium brachygibbosum. Although addition of T.strain P3.9 did not change the diversity of fungi in loquat rhizosphere，but it changed the composition of fungal community. Although the fungal community hardly changed from 10 to 30 days after adding T. strain P3.9，but it changed from 40 to 90 days. The addition of T. strain P3.9 into loquat rhizosphere soil led to the reduction of 2 species of rhizosphere fungi within 90 days. On the one hand，the abundance of dominant genera Mortierella and Trichoderma. and the non-dominant genus Cladophialophora increased significantly. The abundance of dominant species Mortierella alpina and M. exigua also increased significantly. On the other hand，the abundance of dominant genera of Phoma，Aspergillus and Lectera and the 9 non dominant genera decreased significantly. The abundance of 10 non-dominant species also decreased significantly. In conclusion，adding T.strain P3.9 had no adverse effect on the diversity of fungi in loquat rhizosphere. On the contrary，it can improve the richness of species of beneficially fungal communities，and reduce the richness of species of harmfully fungal communities. It has potential remediation effect and fertility enhancement of rhizosphere soil for loquat continuous cropping.