A constant temperature shaker is used to pre-treat the available sulfur in the soil，and the inductively coupled plasma emission spectrometer is used for testing to establish a new detection method for soil available sulfur. The result shows that this method has good accuracy in determining soil available sulfur，and the results of soil reference materials are within the guaranteed value range. The detection limit of this method for soil available sulfur is 0.5 mg/kg. The relative standard deviation(n=6)of the certified standard soil samples is between 2.47% and 4.59%. Through the contrast test of actual cultivated soil in different areas，the relative deviation of the available sulfur between the comparison test and the standard method is from 0.63% to 8.38%. This method is suitable for the detection of available sulfur in different types of soils，and the results are accurate，reliable，simple and quick，and are especially suitable for the detection of large batch soil samples.