The P-dissolving bacteria in rhizosphere soil of Paris polyphylla var. yunnanensis was screened in order to improve the yield and quality of Paris polyphylla var. yunnanensis，and provide excellent strains for the development of microbial fertilizer. The rhizosphere soils of wild and transplanted Paris polyphylla var. yunnanensis in 10 different regions of Sichuan，Yunnan and Guizhou were collected and screened to isolate and screen high-efficiency inorganic P-dissolving bacteria. The P-dissolving capacity was determined by the molybdenum-antimony-scandium colorimetric method，combined with physiological，biochemical and 16S rDNA identification analysis. The results showed that a total of 42 strains of phosphate solubilizing bacteria were isolated and purified. The available phosphorus content in the fermentation broth was 66.68 ～ 104.10 mg/L. Among them，the Z3-4 strain belonging to the genus Bacillus had the strongest ability to solubilize inorganic phosphorus，and was excellent in fermentation. The content of available phosphorus in the clear liquid was 104.10 mg/L，the amount of phosphorus increased was 38.55 mg/L，and the phosphorus solution rate was 37.03%. It can be concluded that strain Z3-4 is a bacterium with high efficiency of dissolving inorganic phosphorus and can be used in the production of biological fertilizer.