Arbuscular mycorrhizal fungi，as the most widespread symbionts in earth，play an important role in all kinds of adversity environment. This study analyzed the effects of molybdenum pollution on the infection，reproduction and the ability of glomalin secretion by arbuscular mycorrhizal fungi in the molybdenum mining areas with different levels of molybdenum pollution. The results showed that，the formation of the symbiotic relationship between arbuscular mycorrhizal fungi and plants was not affected by molybdenum pollution under 4 different levels. The average mycorrhizal infection frequency was 50.64%. The average spore density of soil in Luoyang molybdenum mining area was 19.20 spores·g-1 airdried soil，among which the average spore density of herb and woody plant rhizosphere soil was 18.2 and 20.3 spores·g-1 airdried soil，respectively. Under severe level of molybdenum pollution，the content of total and easily extractable glomalin in soil were significantly lower than those of nonmolybdenum contaminated soil，with the value of 1.53 and 0.72 mg·g-1，respectively. There was a significantly negative correlation between molybdenum content and spore density or glomalin，but there was no significant correlation with mycorrhizal infection rate according to the results of correlation analysis. At the same time，there was no significant correlation between the glomalin and the mycorrhizal infection rate under the other three molybdenum pollution levels except for the light molybdenum pollution level. It can be seen that although molybdenum pollution does not affect the infection of arbuscular mycorrhizal fungi on plants，it has a certain inhibitory effect on the ability of arbuscular mycorrhizal fungi to multiply and secrete glomalin.