海藻提取物缓解高温对樱桃萝卜胁迫效应的研究
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(1.土肥资源高效利用国家工程实验室,山东农业大学资源与环境学院,山东 泰安 271018;2.养分资源高效开发与综合利用国家重点实验室/金正大生态工程集团股份有限公司,山东 临沭 276700;3.众德肥料(平原)有限公司,山东 平原 253100)

作者简介:

王伟涛(1995-),硕士研究生,研究方向为新型肥料研制与应用。E-mail:347939715@qq.com。

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基金项目:

基金项目:山东省重点研发计划项目(2019GNC106011);山东省研究生导师指导能力提升项目(SDYY18108);国家重点研发计划项目(2017YFD0200706)。


Ameliorative effects of seaweed extracts application on heat tolerance of cherry radish(Raphanus sativus L. var. radculus pers)
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(1.National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources,College of Recourses and Environment,Shandong Agricultural University,Tai’an Shandong 271018;2.State Key Laboratory of Nutrition Resources Integrated Utilization/Kingenta Ecological Engineering Group Co.,Ltd.,Linshu Shandong 276700;3.Fertilizer (Pingyuan)Ltd.,Pingyuan Shandong 253100)

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    摘要:

    选取樱桃萝卜(Raphanus sativus L. var. radculus pers)为研究对象,在盆栽试验条件下以清水处理为对照(CK),并以浓度为 66.7 mg·L-1的芸苔素内酯为阳性对照(BHG),通过添加不同浓度(100.0、200.0 mg·L-1)的海藻提取物处理(SWE-1、SWE-2),探究了在高温胁迫(30℃)条件下海藻提取物对樱桃萝卜产量、果实品质、内源激素水平以及抗氧化酶系统的影响。结果表明,SWE-1、SWE-2均可有效缓解高温对樱桃萝卜生长造成的胁迫效应,以SWE-2处理效果最好。与CK处理相比,SWE-2处理的果实鲜重显著提高180.0%,可溶性蛋白含量显著提高35.2%;其叶片的SPAD值显著提高16.8%,硝酸还原酶活性显著提高57.7%,生长素含量显著提高145.4%,而脱落酸含量显著下降42.0%,丙二醛含量显著下降59.2%,谷胱甘肽还原酶活性提升15.7%,抗坏血酸过氧化物酶活性显著上升9.4%。与BHG处理相比,SWE-2处理的果实鲜重显著提高43.5%;可溶性蛋白含量显著提高49.6%,维生素C含量显著提高68.4%;其叶片硝酸还原酶活性显著提高17.1%,谷胱甘肽还原酶活性显著提高23.7%,抗坏血酸过氧化物酶活性显著提高29.9%,过氧化氢酶活性显著提高503.1%,而丙二醛含量显著下降41.7%。这表明海藻提取物能有效提高樱桃萝卜对高温胁迫的抵御能力并且效果优于芸苔素内酯。施用海藻提取物在显著提高樱桃萝卜对高温胁迫抵抗力的同时还显著提升了其产量和品质。海藻提取物获取方式简单、成本低廉,本研究为海藻提取物在果蔬安全生产上的科学施用提供了理论支撑。

    Abstract:

    In order to investigate the effects of seaweed extracts on plant under heat(30℃)stress,the yield,fruit quality,hormone levels,and antioxidant system were analyzed using cherry radish(Raphanus sativus L. var. radculus pers)by pot experiment. Under artificial climate incubator conditions,the cherry radish was incubated in base material under the adding seaweed extracts concentrations of 100.0 and 200.0 mg·L-1( SWE-1,SWE-2 treatment,respectively),using water as control(CK)and the concentration of 66.7 mg·L-1 brassinolide as the positive control (BHG) . The results showed that the seaweed extracts with the concentration of 100 and 200 mg·L-1 could effectively alleviated the stress effect of high temperature on the growth of cherry radish,and treatment with SWE-2 had the best effect. Compared with CK treatment,the fruit fresh weight of SWE-2 treatments was boosted significantly by 180.0%,and the soluble protein contents were boosted significantly by 35.2%; the SPAD readings were boosted significantly by 16.8%,the nitrate reductase activity was boosted significantly by 57.7%,and auxin content was boosted significantly by 145.4%;abscisic acid content was decreased significantly by 42.0%, the malondialdehyde content was decreased significantly by 59.2%,the activity of glutathione reductase was boosted by 15.7%,and the activity of ascorbic peroxidase was boosted by 9.4%. In addition,compared with BHG treatment,the fruit fresh weight of SWE-2 treatment was boosted significantly by 43.5%,the soluble protein content was boosted significantly by 49.6%,and the vitamin C content was boosted significantly by 68.4%. Nitrate reductase activity of leaves was boosted significantly by 17.1%. The malondialdehyde content decreased significantly by 41.7%,the activity of glutathione reductase was boosted significantly by 23.7%,and the activity of ascorbic peroxidase was boosted significantly by 29.9%,the activity of catalase was boosted significantly by 503.1%,which proved that the SWE treatments were more effective than the BHG treatments on alleviating the damage caused by heat stress. In conclusion,the application of seaweed extract can not only significantly enhance the resistance of cherry radish to heat stress,but also significantly enhance its yield and quality. This study provides theoretical support for the scientific application of seaweed extract in the secure production of fruits and vegetables.

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王伟涛,贾春花,张林琳,王小华,孙玲丽,刘之广,张民.海藻提取物缓解高温对樱桃萝卜胁迫效应的研究[J].中国土壤与肥料,2022,(1):188-193.

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  • 收稿日期:2020-09-27
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  • 录用日期:2020-11-30
  • 在线发布日期: 2022-03-09
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