The effects of different root-zone fertilizations of controlled release urea on soil inorganic nitrogen content and growth of ratoon rice were studied to realize simplified fertilization and high-efficiency utilization of nitrogen fertilizer. In this study，5 treatments were set up through field experiments，which were no nitrogen(CK)，farmers’ familiar practice (FFP)，one-time application of controlled release urea at a shallow depth of 5 cm in the root zone(RF1)，one-time application of controlled release urea at a depth of 10 cm in the root zone (RF2)and one-time application of controlled release urea in the root zone layered application fertilization with both 5 and 10 cm depth(RF3). The effects of different fertilization methods on the growth and yield of ratoon rice were analyzed. The results showed that compared with FFP treatment，RF1，RF2 and RF3 significantly increased the NH4+-N content around the root-zone soil，and the NH4+-N content could be maintained at a similar level with FFP for 120 days till the growth period of axillary bud. Among them，the soil NH4+-N of RF3 stratified around the root-zone soil，which could best meet the growth of ratoon rice. Compared with FFP treatment，there was no significant difference in plant height of RF2 and RF3. The dry matter accumulation of RF1，RF2 and RF3 was 3%～8% higher than that of FFP in the first harvest time(the difference between RF3 and FFP was significant)，but there was no significant difference among the fertilization treatments in the second harvest time. Compared with FFP treatment，RF3 treatment significantly increased the nitrogen accumulation of stem, leaf and panicle at full heading stage in the first season and regeneration season. In the first rice season，RF1，RF2 and RF3 improved rice yield by 1% ～ 10% compared with FFP，in which RF3 had significant higher yield than FFP，while in the regeneration season，RF1，RF2 and RF3 had similar rice yield with FFP. In conclusion，the one-time root zone application of controlled release urea can meet the nutrient requirements of regenerated rice in the whole growth period by the concentration and slow release of nitrogen，which is an easy and simplified fertilization model and worthy to popularize，and the root zone layered application of controlled-release urea has the best effect.