In order to reveal the soil carbon(C)，nitrogen(N)，phosphorus(P) spatial distribution characteristics in grassland soils of different latitudes，the grasslands on China-Mongolia border(Erenhot City，East Ujimqin Banner and Xin Barag Right Banner)were selected as the study areas.The spatial distribution characteristics of C，N and P elements in different soil layers(0～10，10～20，20～30 cm)in the grassland were analyzed to discuss the main factors influencing the spatial variation of soil stoichiometric. The results showed that:(1)Within the range of 0～30 cm layer，the contents of organic carbon，total nitrogen and total phosphorus in the soil of the three grassland areas gradually increased with the increase of latitude，which were all shown as Xin Barag Right Banner>East Ujimqin Banner>Erenhot City.The content of organic carbon and total nitrogen in the topsoil of the same area was higher than that in the lower layer，but the variation of total phosphorus content in different soil layers was not significant.(2)There was no significant difference in C/N，C/P，N/P，C/N/P between different soil layers in the same area(P>0.05).The C/N，C/P and C/N/P of same soil layer in different grasslands showed latitude differences. C/N of each layer of soil from high to low was in the order of East Ujimqin Banner>Xin Barag Right Banner>Erenhot City，while C/P of each soil layer showed as Xin Barag Right Banner>East Ujimqin Banner>Erenhot City，however，C/N/P of each soil layer ranked as Erenhot City>East Ujimqin Banner>Xin Barag Right Banner.With the increase of soil depth，N/P in the three areas showed different trends: Erenhot City showed an increasing trend first and then decreased；East Ujimqin Banner showed a gradually decreasing trend；Xin Barag Right Banner showed a decreasing trend first and then increased.(3)Latitude，precipitation，temperature，and soil pH were the important factors that affected the change of soil stoichiometric.C/P and N/P was significantly positively correlated with latitude(P<0.01)，while C/P and N/P were significantly negatively correlated with soil pH，precipitation and temperature (P<0.05).C/N/P had a significantly negative correlation with latitude(P<0.05)，but a significantly positive correlation with soil pH and temperature(P<0.05). Results of the study can provide references for the discussion of soil nutrient balance and sustainable development of grassland ecosystem in different latitudes of the border between China and Mongolia.