The carbonization and fertilizer utilization of kitchen food waste was one of the effective ways to solve the problem of kitchen food waste environmental pollution.At present，there was still lack of research on the fertilizer efficiency of kitchen waste biochar and biochar-based fertilizer，including their impact on the soil environment.The kitchen waste biochar was mixed with compound fertilizer and agricultural waste to prepare the biochar with compound fertilizer(BCF)and biochar with organic fertilizer(BOF)，respectively.A pot experiment was conducted to comparatively study the effects of fertilizers produced from different kitchen waste biochar on soil physicochemical characters and bacterial community structure of mustard planting under the same nitrogen input.The BOF treatment only reduced the vegetable soil pH value by 0.14～0.17 as compared with no fertilization(NF)treatment(control group).Compared with chemical fertilizer(CF)，organic fertilizer(OF)and kitchen biochar(KB)treatment，the soil electrical conductivity(EC)of BCF and BOF treatments increased by 22.34%，36.73%，27.61% and 18.67%，33.74%，24.20%，respectively，which significantly increased the total salt ion content of vegetable soil.Compared with CF，KB and BCF treatments，the dissolved organic carbon(DOC)of OF and BOF treatments increased by 25.33%，22.07%，23.05% and 14.87%，11.15%，12.28%.The dissolved organic nitrogen(DON)of BCF and BOF treatments increased by 16.39% and 7.63% as compared with KB treatment.The BOF treatment could enhance the soil EC，DOC and DON，but without increasing the vegetable soil acidification.Proteobacteri，Actinobacteria，Acidobacteria，Chloroflexi and Bacteroidetes were the dominant bacteria at the phylum level；Sphingomonas，Lysobacter，Arthrobacter，Gemmatimonadaceae，Gaiella，Massilia，Roseiflexaceae and Xanthobacteraceae were the dominant bacteria that could be recognized at the genus level. In addition，there were significant differences in the relative abundance of Arthrobacter，Gaiella and Roseiflexaceae among treatments of NF，CF，OF，KB，BCF and BOF(P<0.05).Compared with other treatments，the BOF treatment could increase the bacteria diversity(except the NF treatment)and abundance of vegetable soil，and significantly increase the number of differential indicator bacteria.Compared with kitchen biochar，chemical fertilizer or organic fertilizer alone，the combination treatment of kitchen biochar and chemical fertilizer or organic fertilizer were more effective in increasing the yield and dry biomass of mustard.The BOF prepared from kitchen waste biochar and organic agricultural waste was added to vegetable soil，which could enhance soil fertility and increase bacterial abundance，and thus had a good prospect for fertilizer utilization.