In order to explore the effects of inoculation and fertilization on the growth of seedlings，the 2-year-old Pinus sylvestris seedlings were studied under different inoculation treatments and fertilization regimes to provide a theoretical basis for precise fertilization of P. sylvestris seedlings.The 8 kinds of ectomycorrhizal fungi were mixed inoculated in the process of seedling transplanting，and no inoculation seedlings was used as the control. Treatments including exponential fertilization(EF)，double exponential fertilization(DEF)，conventional fertilization(CF)，inoculation+exponential fertilization(JEF)，inoculation+double exponential fertilization(JDEF)，inoculation+conventional fertilization(JCF)were set up.To prevent the migration of fungi and the interplay of fertilization，the treatments are separated by PVC partitions.The results showed that the seedling growth index，nutrient accumulation(N，P)and mycorrhizal infection rate of exponential fertilization were significantly higher than those of CF treatment，with the largest for seedlings indexes under JEF treatment.The height，ground diameter and biomass of seedlings under JEF treatment were 1.30，1.42 and 1.34 times that of the mycorrhizal seedlings under JCF treatment，and 1.44，1.55 and 2.01 times that of the non-mycorrhizal seedlings under CF treatment. Compared with JCF treatment，N accumulation increased by 32.7%. Compared with CF treatment by 96.4%，P accumulation increased by 48.6% compared with JCF treatment，and increased by 100.7% compared with CF treatment. Thus，the combinations of inoculation and exponential fertilization treatment were more beneficial to cultivate high-quality P. sylvestris seedlings.