Flue-cured tobacco is an important economiccrop，and continuous cropping obstacle has become one of the mainreasons limiting its industrialdevelopment. The effects of long-term continuous cropping and reductive soil disinfestation(RSD) on the fungal community in rhizosphere soil were studied to provide theoretical basis for alleviating continuous cropping obstacles of flue-curedtobacco.The rhizosphere soil of flue -cured tobacco cropping for3，5 and 7 years was selected toanalyze the physical and chemical properties and the succession law of fungal community.The RSD treatment was applied to the soil of continuous tobacco cropping for 3 years and the repair effect of RSD was evaluated by comparing with untreatedgroup.The results showed that:(1)Continuous cropping significantly reducedpH，the contents of total carbon and total nitrogen in rhizosphere soil of flue-curedtobacco，and RSD treatment significantly increased the contents of total carbonand total nitrogen in 3 year treatment.(2)The results of sequencing showed that continuous cropping treatment significantlyincreased the Alpha diversity of fungalcommunity.Principal axis analysis and cluster analysis showed that continuouscropping significantly changed the structure of fungalcommunity，the relative abundance of fungal community and thecomparison results of FUNGuild database showed that continuous cropping treatment increased the abundance of pathogens.(3)Compared with untreated 3 years treatment，RSD treatment significantly reduced fungal community diversity and pathogen abundance.(4)Correlation heat map analysis showed that the relative abundance of pathogens was significantly negativelycorrelated withpH，total carbon and total nitrogencontent.Therefore，long -term continuous cropping of flue-cured tobaccoresulted in the degradation of rhizosphere soil quality and the increase ofpathogens.RSD treatment had good remediation effect on soil quality and fungal community.