In order to explore the physiological role ofglutathione(GSH)in pepper response to cadmiumstress，the effectsof GSH and GSH synthesis inhibitor buthionine sulfloximine(BSO)on various physiological indexes of pepper leaves androots were determined under cadmium stress.The results showed that 100μmol/L cadmium ions seriously inhibited thegrowth of pepperseedlings.Spraying 10 mmol/L GSH under cadmium stress increased the rootlength，plantheight，freshweight of underground parts and aboveground parts of pepper seedlings，which increased by22.01%，6.59%，39.11%and26.47%，respectively，compared with Cd -only treatment；The contents of chlorophyll a，chlorophyll b and carotenoidincreased by82.65%，51.33% and32.19%，cadmium content in roots and leaves increased by 11.26% and 33.26%respectively；The activities of superoxide dismutase，catalase，ascorbate peroxidase，peroxidase，dehydroascorbatereductase，dehydroascorbate reductase，glutathione reductase and glutathione peroxidase in roots increased by49.63%， 68.15%，51.60%，59.72%，56.78%，17.48%，59.53% and23.27%，respectively；while in leaves increased by14.22%，28.11%，64.86%，40.47%，28.02%，14.30%，28.60% and47.46%，respectively；The ratio of reduced state to oxidizedstate in GSH circulation system increased by 93.78% and 40.32% in leaves and roots，respectively，thus reducing the production rate of superoxide anion and the content of H2O₂.AddingBSO，an inhibitor of GSHsynthesis，would reverse theaboveeffects.GSH can effectively alleviate the physiological damage of pepper caused by cadmiumstress，and then improve the ability of pepper to resist heavy metal stress.