The endophytic bacterial HMC50 was isolated from rice roots and identified as paraburkholderia kururiensis by 16SrRNA gene and whole genomicanalysis.The strain was predicted to have the main features associated with the promotion ofplantgrowth，flagella -mediated motility and detoxification at the genomiclevel.In the predictionresults，18 genes related to nitrogen fixation were found to include the key gene nifH，as well as tryptophan synthase alphachain(EC4.2.1.20)andtryptophan synthase beta chain(EC4.2.1.20)genes，so it is speculated that this strain could fix N 2and secreteIAA. The results of experiment on plant growth-promoting properties verified that HMC50 had the ability to secreteIAA(29.57mg/L)and had a high nitrogenase activity of 11.9nmol/(mL·h).Inaddition，this strain also had the ability to producesiderophores，and the quantitative detection A/Ar was0.29，the activity of ACC deaminase was 0.35U/mg，and the abilityof dissolve inorganic phosphorus is strong，and the ratio of the diameter of the transparent circle phosphorus-soluble of colonyto the diameter of the colony is 3.97.The pot experiment showed that the strain had the potential to promote the growth ofrice，and it was better than the blank control group in terms of rootlength，stemlength，root weight and total fresh weight ofseedlings(P<0.05).The above results show that strain Paraburkholderia kururiensis HMC50 has excellent plant beneficialcharacteristics.The results are helpful for the development of microbial fertilizer for rice.