Samples of lily plants-Fangio were collected from the Lily Resource Nursery in Fangshan District，Beijing，and some endophytic bacteria were isolated by the method of tissue block separation；Antagonistic strains with antagonisticactivity against the strains of several major lily pathogens were screened by the plate confrontation method，using lily gray mold-Botrytis cinereaACCC36423，lily leaf wilt-Botryosphaeria dothidea ACCC37263 and Fusarium oxysporum F01139and other major lily pathogenic fungi as the indicator bacteria.The antagonistic strains were tested for the production of 1-aminocyclopropane-1-carboxylicacid(ACC)deaminase，production of indole aceticacid(IAA)，phosphatesolubilization，production of siderophore，and then the strain was identified preliminarily according to its morphologicalobservation and the sequence analysis of 16S rRNAgene.An endophytic bacterial strain named FJb -2 was isolated fromthe stem oflily；the strain FJb -2 showed the high antagonistic activity against Botrytis cinerea，Botryosphaeria dothidea and Fusarium oxysporum，and the growth inhibition rate of pathogenic fungi ranged from 60% to81%.This strain alsohad ACC deaminase activity of(35.28±0.05)μmol α -ketobutyric acid/(mg·h)and had some plant growth -promotingcharacteristics such as IAAproduction，phosphorus solubilization and siderophoreproduction.The FJb -2 strain was preliminarily identified as a Burkholderia bacterium based on the 16S rRNA gene sequenceanalysis.It is concluded that the lily endophytic bacterium Burkholderiasp.FJb-2 has a highly effective antagonistic effect against a variety of lily pathogenic fungi under in vitroconditions，and has the potential to promote plant growth.