The application of slow and controlled-release nitrogen fertilizer is one of the common measures to reduce the loss of ammonia volatilization in paddy soil.Combining slow and controlled-release nitrogen fertilizer with quick acting nitrogen fertilizer can solve the contradiction between rice demand for nitrogen and reducing nitrogen loss. In order to reduce ammonia volatilization loss，improve nitrogen use efficiency and reduce environmental pollution on the premise of ensuring rice yield，field split plot experiment was adopted.In order to study the effects of different nitrogen application rates and nitrogen application methods on ammonia volatilization，nitrogen use efficiency and rice yield，five nitrogen application levels of 60 (N60)，120 (N120)，180 (N180) and 240 (N240) kg·hm-2 were set up，and two fertilization methods of base application(SF) and half fertilizer applying by base application with twice topdressing of the other half (TF) were used. The results showed that nitrogen application methods and nitrogen rates had significant effects on ammonia volatilization in rice field.Under the same nitrogen application mode，ammonia volatilization loss of paddy soil increased with the increase of nitrogen application rate.The ammonia volatilization loss of SF was 14.46～23.74 kg·hm-2，while that of TF was 23.3～47.74 kg·hm-2，and the ammonia volatilization loss of SF was 37.9%～50.3% lower than that of TF.The apparent nitrogen use efficiency and nitrogen partial productivity were significantly affected by nitrogen application methods.The maxium apparent nitrogen use efficiency of SF and TF appeared at N180，50.02% and 38.68%，respectively.The partial nitrogen productivity of TF was higher than that of SF at low nitrogen application rate (N60) and high nitrogen application rate (N240)，while the partial nitrogen productivity of SF was higher than that of TF at 120 (N120) kg·hm-2 and 180 (N180) kg·hm-2 by 3.32 and 5.58 kg·kg-1，respectively.The nitrogen application rate significantly affected the nitrogen uptake and nitrogen agronomic use efficiency of rice.The highest yield of SF and TF appeared in N180 and N240，respectively，and SF was higher than TF，with a difference of 465.3 kg·hm-2.The combined application of slow controlled and release nitrogen fertilizer and quick acting nitrogen fertilizer can reduce the ammonia volatilization loss of rice field effectively，improve the apparent utilization rate and partial productivity of nitrogen，and achieve higher yield under the condition of low nitrogen application，which is of practical value in the management of nitrogen fertilizer in rice.