To explore the efficient nitrogen application method for japonica rice production in rice-vegetable rotation，improve the utilization efficiency of nitrogen fertilizer，and provide theoretical basis for realizing the green production goal of reducing nitrogen and increasing efficiency，field experiments were conducted with two conventional japonica rice varieties (Huijing 17 and Chujing 28) as materials in Yunnan in 2016 and 2017，and the effects of different nitrogen application techniques on rice growth and yield formation were compared.Four treatments with different nitrogen rates and ratios of basal，tiller，spikelet-promoting and spikelet-sustaining application (N0，0 kg/hm²； CK，total N 270 kg/hm²，5∶5∶0∶0； BNF，total N 216 kg/hm²，2.5∶2.5∶2.5∶2.5； FCBP，total N 162 kg/hm²，0∶0∶6∶4)were set up.Rice population characteristics difference in growth stage，yield and its components at maturity，grain/leaf ratio，main population quality indices were determined.Compared with CK，yield was significantly increased under BNF and FCBP，which increased by 19.23% and 21.93% in 2016，11.70% and 27.04% in 2017，respectively. Nitrogen agronomic efficiency was greatly improved，which was increased by 165.20% and 280.45% under BNF and FCBP in 2016，and by 105.02% and 313.42% in 2017，respectively.Mechanism of high yield and apparent nitrogen recovery efficiency in FCBP was that high ratio of productive tillers to total tillers and enough population panicles were formed through controlling non-productive tiller and reducing the peak seedling number of the population； high yield population quantity were obtained through forming large total number of spikelets，high grain/leaf ratio，appropriate LAI and high effective leaf area exceeded 80% were enlarged through enriching dry matter accumulation from heading to maturity stage.Overall，FCBP had provided a technical support for rice green production in Yunnan rice rotation.