In order to explore the effect of earthworm dung and coconut bran composite substrate on the growth of Panax notoginseng seedlings，a single factor randomized block design was adopted.There were 12 treatments，including earthworm dung group(T1～T4)，coconut bran group(T5～T8)，earthworm dung + coconut bran group(T9～T11)，and the traditional substrate Peat∶vermiculite = 2∶1(V∶V) was used as the control group(CK).Combined with the comprehensive evaluation of correlation and membership function method，the matrix formula suitable for the growth of Panax notoginseng seedlings was selected objectively and effectively.The results showed that the coconut bran group had smaller bulk density and larger porosity； the ratio of big pore size to small pore size was close to the optimal value of 0.5(T5 equal to 0.5)； the pH was within the suitable range for the growth of Panax notoginseng seedlings； the content of organic matter was high，and the total nitrogen，total phosphorus，available phosphorus and alkali hydrolyzable nitrogen were similar to those of CK.The comprehensive ranking of membership function values of T5，T6，T7 and T8 was higher than that of CK，and T5 ranked the highest.The root fresh weight，shoot fresh weight，ratio of root to shoot，seedling index and G value of T5 were better than those of other treatments，which increased by 34.84%，12.50%，14.58%，84.05% and 37.50%，respectively.The results showed that T5[coconut bran∶peat∶vermiculite∶raw soil = 0.5∶1.5∶1∶1(V∶V)]was more suitable for the growth of Panax notoginseng，and could be used as the compound matrix formula of Panax notoginseng seedling.