硅酸钠对苗期甜菜形态和生理生化特性影响的研究
作者:
作者单位:

(1.黑龙江大学生命科学学院,黑龙江 哈尔滨 150080;2.黑龙江大学现代农业与生态环境学院,黑龙江 哈尔滨 150080)

作者简介:

刘钰(1997-),硕士研究生,研究方向为植物生态学。E-mail:15734589866@163.com。

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基金项目:

基金项目:国家自然科学基金(31701487);国家糖料产业技术体系“甜菜种植制度”(CARS-170209);中国博士后科学基金(2020M670944);黑龙江省自然科学基金(YQ2020037C)。


Effect of sodium silicate on the morphology and physiological and biochemical characteristics of sugar beet
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(1.College of Life Science,Heilongjiang University,Harbin Heilongjiang 150080;2.Modern Agriculture and Ecological Environment Institute,Heilongjiang University,Harbin Heilongjiang 150080)

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    摘要:

    硅在植物生长发育和抵御逆境等过程中均发挥着重要作用。为了解不同浓度硅元素对苗期甜菜生长的影响,为后续利用外源硅调节植物生长奠定理论基础。以KWS1176甜菜品种为研究对象,在培养液中含有适合甜菜生长的3 mmol·L-1 Na+的条件下,设置0、0.3、0.6、1.2、2.4和4.8 mmol·L-1 6个浓度梯度硅酸钠溶液施加至培养液中,测定不同处理对甜菜幼苗形态特征以及生理生化指标的影响。结果表明:施硅对甜菜幼苗的生长起到明显促进作用,能显著增加甜菜幼苗的株高并使叶片较对照直立。同时,适宜浓度的硅酸钠能降低甜菜植株丙二醛(MDA)含量,降低超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)以及过氧化物酶(POD)活性,以CAT活性降低最为显著。此外,硅能够促进甜菜叶片光合作用,气孔导度(Gs)、蒸腾速率(Tr)、净光合速率(Pn)在硅酸钠的作用下均有所提高,而胞间CO2浓度(Ci)则呈递减趋势。通过主成分分析筛选出干重、鲜重以及脯氨酸含量等6个指标,可作为指示性指标用于评价硅酸钠对甜菜幼苗形态和生理生化特性影响的强弱。计算不同浓度下甜菜叶片和根系的第一和第二主成分综合得分,并对其进行排名,最终得到当硅浓度为2.4 mmol·L-1时,甜菜根系和叶片的生长及生理指标最优。

    Abstract:

    Silicon plays an important role in plant growth and resistance to adversity.In order to understand the effects of different concentrations of silicon on growth of sugar beet seedlings,thus laying a foundation for studying the regulation of silicon on plants,the indoor hydroponics experiment was conducted to study the morphological characteristics and physiological and biochemical changes of KWS1176 sugar beet varietiy,based on 3 mmol·L-1 Na+,under six concentration gradients of sodium silicate solution,including 0,0.3,0.6,1.2,2.4 and 4.8 mmol·L-1.The results showed that silicon significantly promoted the growth of sugar beet seedling,increased the height of sugar beet seedlings and made the leaves more erect than the control.At the same time,the appropriate concentration of sodium silicate reduced the malondialdehyde(MDA) content of sugar beet plants,and reduced superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX) and peroxides enzyme(POD) activity.It is worth mentioning that among the four enzymes,CAT activity decreased most significantly.In addition,silicon promoted photosynthesis of sugar beet leaves.Stomatal conductance(Gs),transpiration rate(Tr),and net photosynthetic rate(Pn) were all increased under the action of sodium silicate,while the intercellular CO2 concentration(Ci) showed a decreasing trend.Six indicators including dry weight,fresh weight and proline content etc.,were screened out by principal component analysis,which can be used as indicative indicators to evaluate the effect of sodium silicate on the morphology and physiological and biochemical characteristics of sugar beet seedlings.The comprehensive scores of the first and second principal components of sugar beet leaves and roots at different concentrations were calculated and ranked.When silicon concentration is 2.4 mmol·L-1,the growth and physiological indexes of sugar beet roots and leaves are the best.

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刘钰,范国凯,刘丹阳,於丽华,王宇光,耿贵.硅酸钠对苗期甜菜形态和生理生化特性影响的研究[J].中国土壤与肥料,2022,(5):124-133.

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  • 收稿日期:2021-02-07
  • 最后修改日期:
  • 录用日期:2021-02-23
  • 在线发布日期: 2022-07-08
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