In order to solve the problems of large amount of strawberry fertilizer input，many times of top dressing and high labor cost under the condition of substrate cultivation，controlled-release nitrogen fertilizer was used instead of water-soluble fertilizer to realize one-time fertilization of strawberry. Taking the “Hongyan” strawberry as the test material and the conventional optimal fertilization treatment as the control，the effects of the optimal fertilization treatment and the controlled-release fertilizer treatment with different nitrogen dosages on the yield，quality and economic benefit of the strawberry under the conditions of substrate cultivation were determined，and the optimal fertilization treatment was analyzed and calculated nitrogen content. The results showed that，compared with conventional optimal fertilization treatment，the controlled-release nitrogen fertilization treatment increased the yield，single fruit weight，economic benefit，sugar content and Vc content by 7.4%～26.5%，9.1%～33.3%，7.5%～26.7%，7.7%～31.4% and 8.8%～14.0%，respectively. Except for the controlled-release nitrogen fertilizer nitrogen dosage 97.50 kg/hm² treatment，partial factor productivity from applied nitrogen，agronomic efficiency of applied nitorgen increased by 26.5%～144.3%，73.7%～222.7% compared with coventional optimal fertilization treatment. In conclusion，the substitution of controlled-release nitrogen fertilizer for water-soluble fertilizer not only reduced the amount of fertilizer，guaranteed the yield of strawberry，but also achieved high yield，and improved the economic benefit of strawberry. By fitting the relationship between the amount of controlled-release fertilizer and strawberry yield，single fruit weight with quadratic curve，the optimal recommended amount of controlled release nitrogen fertilizer was about N 60 kg/hm²，which provided reference for practical production. To sum up，the replacement of water-soluble fertilizer with controlled-release nitrogen fertilizer improves the yield of strawberries，ensures the quality of strawberries，reduces the cost of fertilizer input and improves economic benefits. Considering the yield，single fruit weight，quality，fertilizer productivity and economic benefits of strawberry，the recommended amount of controlled-release nitrogen fertilizer for strawberry production is about N 60kg/hm².