In order to understand the bioavailability of selenium in selenium ore powders，a pot experiment applying selenium ore powders in soil at the rates of 0，3，5，10，20，25 and 50 g/kg to cultivate radish seedlings for two months was conducted and selenium content，the biomass，the contents of vitamin C(Vc)，soluble sugar，malondialdehyde(MDA)，photosynthetic pigments and the catalase(CAT)activity in the leaves of radish seedlings were analyzed. The results showed a significant positive correlation between the soil selenium ore powder application rates and the selenium content of radish seedlings，but the biomass of radish seedlings was decreased by selenium ore powder applications. The application of 10 g/kg selenium ore powder increased 70% of the Vc and 50% of the soluble sugar content of radish seedlings compared with the control，but the highest application of 50 g/kg significantly reduced the Vc and soluble sugar content. Excluding the control，there was a significant positive correlation between the CAT activity of radish seedlings and the soil selenium ore powder applications. The MDA content of radish seedlings was significantly reduced by the selenium ore powder applications of 10 and 25 g/kg. Appropriate selenium ore powder applications increased the photosynthetic pigment content of radish seedling leaves with the highest content at 10 g/kg，while the highest application rate of 50 g/kg reduced the photosynthetic pigment content significantly. Except for 25 g/kg，the soil selenium ore powder applications reduced the ratio of chlorophyll a to chlorophyll b significantly. There was a significantly linear correlation between the content of photosynthetic pigment and the content of soluble sugar of radish seedling leaves under the condition of soil application of selenium ore powder. In conclusion，the selenium content and CAT activity were increased by soil selenium ore powder applications，and the content of Vc，soluble sugar and photosynthetic pigments were increased and MDA was reduced in radish seedling leaves by proper applications，indicating that of selenium ore powers had effects on the physiological and biochemical metabolism processes and the growth of radish，and this confirmed the selenium bioavailability in selenium ore powders，but the effect of selenium ore powder on the biomass of radish seedlings needs to be further studied. The results provide a scientific foundation for using selenium ore as a selenium resource in agriculture.