In this study，the effects of combined application of humic acid with seedling raising substrate on seedling quality，nutrient absorption characteristics and rice yield were analyzed by using the filed seedling tray and field experiment methods. Rice variety of Huiliangyou 882 was used as the test rice seeds material. The substrate used for rice seedlings was composed of decayed straw，decayed rice husk，vermiculite and soil in volume ratio of 3∶2∶2∶3. Five treatments，including seedling substrate without fertilization(CK)，seedling substrates with humic acid(HA)，seedling substrates with phosphate and potassium fertilizer(PK)，seedling substrates with nitrogen，phosphorus and potassium fertilizers(NPK)，seedling substrates with nitrogen，phosphorus and potassium fertilizers and humic acid(HNPK)were conducted in this study. The rapid increase of rice seedling plant height，shoot dry weights，root morphological indexes and nutrient accumulation in seedling plant were found at the initial growing stage，and then a slow increase at the following growing stage. The above-ground weight，root morphological indexes and plant nutrient accumulated in seedlings in the treatments of NPK and HNPK were significantly higher than that in the CK at 10th day growing stage(P<0.05). Under the same quantity of NPK fertilization，the vigorous seedling indexes of rice in HNPK were increased by 8.58% than that in the treatment of NPK at the 20th day，and the nitrogen，phosphorus and potassium accumulated in rice seedling in treatment of HNPK were increased by 3.65%～13.12%，3.76%～16.06% and 4.22%～13.12% than that in the treatment of NPK，respectively，which showed the significant differences(P<0.05)at the 15th day. The number of effective ear panicles and grains per panicle in the treatment of HNPK were 2.63% and 1.19% higher than that of NPK，respectively，and the rice yield increased by 4.30% than the latter，which showed the significant differences(P<0.05). In summary，the combined application of chemical fertilizer of NPK and humic acid with the seedling raising substrate improved seedling quality，nutrient absorption and accumulation in rice seedling，and increased the number of productive ear per plant and rice yield.