丛枝菌根真菌对镉胁迫下黑麦草光合生理的响应
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(广安职业技术学院,四川 广安 638000)

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韩玮(1984-),讲师,本科,主要从事土壤肥料与生态环境研究。E-mail:175005442@qq.com。同为通讯作者。

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基金项目:四川景观与游憩研究中心资助项目(JGYQ2021017)。


Response of arbuscular mycorrhizal fungi on photosynthetic physiology of Lolium perenne under Cd stress
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(Guang′an Vocational and Technical College,Guang′an Sichuan 638000)

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    摘要:

    为探究镉(Cd)胁迫下丛枝菌根(AM)真菌对黑麦草(Lolium perenne)光合生理的影响,盆栽条件下以Cd浓度为0、5、15和30 mg·kg-1分别对黑麦草接种AM真菌摩西斗管囊霉(FM)、变形球囊霉(GV)、混合处理(FM+GV)以及不接种对照(NM)共16个处理。结果表明,Cd降低了AM真菌侵染;接种AM真菌则提高了黑麦草株高、叶绿素含量、荧光参数、地上部和根系氮(N)、Cd含量,增强光合作用,显著提高植物体内生理活性。在Cd浓度为30 mg·kg-1水平下,FM+GV处理的菌根侵染率最大,为42.7%,与NM相比,FM+GV处理的黑麦草叶片叶绿素含量、地上部和根系N含量分别提高26.2%、70.6%和85.3%;株高和地上干重分别提高34.1%和18.8%;PSⅡ最大光化学效率和PSⅡ潜在活性分别提高4.9%和19.7%;净光合速率、蒸腾速率和气孔导度分别增加19.8%、28.3%和14.7%;超氧化物歧化酶、过氧化物酶活性、可溶性蛋白含量分别是NM的1.3、1.5和1.8倍,丙二醛含量相较NM下降50.0%。土壤中Cd浓度与植株地上部和根内的Cd含量呈显著正相关,FM+GV处理的黑麦草地上部和根系Cd含量分别达到88.1和606.7 mg·kg-1。总体来看,FM+GV处理增加黑麦草光合生理抗性、吸收固持Cd能力的效果最为显著。

    Abstract:

    In order to explore the effect of arbuscular mycorrhizal (AM) fungi on photosynthetic physiology of Lolium perenne under Cd stress,a total of 16 treatments were designed with L.perenne inoculated with or without AM fungi Funneliformis mosseae(FM),Glomus versiforme(GV),mixed treatment(FM+GV) and control treatment (NM) in the soil added with Cd content of 0,5,15 and 30 mg·kg-1,respectively.The results showed that Cd treatment reduced the infection of AM fungi.Inoculation with AM fungi significantly increased the plant height,chlorophyll content,fluorescence parameters,N and Cd content in shoots and roots of L.perenne,enhanced photosynthesis and chlorophyll content of plants,and significantly increased the physiological activity of plants.Under the level of 30 mg·kg-1 Cd,the mycorrhizal infection rate of FM+GV treatment was 42.7%,compared with the control,the chlorophyll content,N content in shoot and root in the leaves of L.perenne increased by 26.2%,70.6% and 85.3%;plant height and aboveground dry weight increased by 34.1% and 18.8%;the PS Ⅱ maximum photochemical efficiency and PS II potential activity increased by 4.9% and 19.7%;and the net photosynthetic rate,transpiration rate and gas pore conductivity increased by 19.8%,28.3% and 14.7%;respectively.The activities of superoxide dismutase,peroxidase,soluble proline were 1.3,1.5 and 1.8 times as compared with the control,but the malondialdehyde content decreased by 50.0%.There was a significanty positive correlation between Cd concentration in soil and Cd content in shoot and root,the Cd content in the top and root of L. perenne inoculated with FM+GV reached 88.1 and 606.7 mg·kg-1,respectively.In general,FM+GV treatment had the most significant effect on increasing photosynthetic physiological resistance and Cd absorption and fixation capacity of L.perenne.

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韩玮.丛枝菌根真菌对镉胁迫下黑麦草光合生理的响应[J].中国土壤与肥料,2022,(6):204-212,220.

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  • 收稿日期:2020-10-27
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  • 录用日期:2021-01-28
  • 在线发布日期: 2022-08-08
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