The application of organic fertilizer is one of the important measures to control the yield and quality of tea.The substitution of organic fertilizer for chemical fertilizer helps to increase the yield of tea with high quality，improving the soil physical and chemical properties and soil environment.The effects of different proportions of bioorganic fertilizer (F) substitution on lu’an Guapian tea growth，intrinsic chemical quality，sensory quality and soil fertility were studied through 2-year continuous field experiments.The results showed that 50%F and 70%F had the best performance in the weight of 100 buds and the density of bud tea，which could increase the yield of tea，and sensory evaluation was also relatively good.The contents of free amino acids and water extracts in tea showed a trend of rising first and then reducing with the increase of the application amount of bio-organic fertilizer.While tea polyphenols and phenol-ammonia ratio showed the opposite trend，which decreased first and then increased.The caffeine showed a trend of increasing successively.The best improvement of tea content was 50%F substituting for chemical fertilizer.The soil pH and contents of organic matter，available phosphorus，available potassium increased with the increase of the application amount of bio organic fertilizer.Bio organic fertilizer substituting for 50% chemical fertilizer improved the weight of 100 buds，the density of bud tea，the quality of tea leaves and soil fertility，the next dispose is 70% substituting for chemical fertilizer.The results can provide a reference for improving the quality and efficiency of tea.