芽孢杆菌与根瘤菌复合包衣对大豆结瘤固氮能力的影响
作者:
作者单位:

(1.中国农业科学院农业资源与农业区划研究所,北京 100081;2.农业农村部微生物产品质量安全风险评估实验室,北京 100081)

作者简介:

赵宇宾(1989-),助理研究员,博士,主要从事复合根瘤菌群的筛选及生态效应研究。E-mail:282236083@qq.com。

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基金项目:

基金项目:云南省重大科技专项计划——云南高原特色经济作物专用生物肥料创制及产业化应用(202202AE090025);国家重点研发计划(2021YFD1700200);中国农业科学院重大联合攻关任务——大豆根瘤菌长效包衣技术(CAAS-ZDRW202003-10);财政部和农业农村部国家现代农业产业技术体系(CARS-04);中国博士后科学基金资助项目(2021M693450)。


Effect on the nodulation and growth of soybean coated with Bradyrhizobium and Bacillus co-inoculants
Author:
Affiliation:

(1.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;2.Laboratory of Quality & Safety Risk Assessment for Microbial Products( Beijing),Ministry of Agriculture and Rural Affairs,Beijing 100081)

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    摘要:

    为了探究不同芽孢杆菌与根瘤菌复合包衣对大豆结瘤固氮的影响,通过盆栽试验,设置不接菌对照(CK)、根瘤菌单独包衣(R)、阿氏芽孢杆菌(RB)和胶质类芽孢杆菌(RP)2种促生菌分别与根瘤菌双包衣以及 3种菌混合包衣(RBP)共5个处理,测定大豆根瘤数量、干重、根瘤固氮酶活性及植株氮、磷、钾含量,筛选根瘤菌剂最佳组合。结果表明,接种菌剂的处理均能够促进大豆结瘤固氮,其中RB处理效果最好,能够获得最高的大豆植株干重、根瘤数量、根瘤干重、固氮酶活性、全氮含量,比CK分别提高51.80%、14.44%、53.33%、44.36%、25.61%,3种菌包衣并没有比双接种促生效果更好。相关性分析表明,根瘤干重与固氮酶活性、根瘤数量及植株干重都呈显著正相关关系,说明根瘤的质量可能比数量更能准确评价共生固氮效率。此结果为根瘤菌剂应用于机械化种植提供可能性,为进一步推广根瘤菌剂的规模化生产应用提供了科学依据。

    Abstract:

    To explore the effect on the nodulation and growth of soybean when coated with Bradyrhizobium and Bacillus co-inoculants,pot experiment was carried out.Five treatments were set up,including CK(no inoculant),R(Bradyrhizobium inoculant coating),RB(co-inoculant coating of Bradyrhizobium and Bacillus aryabhattai),RP(co-inoculant coating of Bradyrhizobium and Paenibacillus mucilaginosus)and RBP(multi-inoculant coating of Bradyrhizobium,Bacillus aryabhattai and Paenibacillus mucilaginosus).Soybean nodule number,nodule dry weight and nodule nitrogenase activity,total nitrogen,phosphorus and potassium of soybean plant were analyzed.The result showed that all treatments with inoculation growth-promoting bacteria promoted soybean nodulation.The RB treatment obtained the highest soybean plant dry weight,nodule number,nodule dry weight,nitrogenase activity,and total nitrogen content,which were higher than that of CK by 51.80%,14.44%,53.33%,44.36% and 25.61%,respectively,and the three kinds of bacterial coating were not better than double inoculation in growth-promoting.Correlation analysis showed that nodule dry weight was significantly positively correlated with nitrogenase activity,nodule number and plant dry weight,indicating that the quality of nodule was more accurate than the quantity to evaluate the symbiotic nitrogen fixation efficiency.The study optimized a high-efficiency composite Bradyrhizobium and Bacillus coating agent for soybean,and provided a scientific support for the application of Bradyrhizobium in the future.

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赵宇宾,关大伟,邢鹏飞,王天舒,马鸣超,姜昕,李俊.芽孢杆菌与根瘤菌复合包衣对大豆结瘤固氮能力的影响[J].中国土壤与肥料,2022,(7):222-227.

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  • 收稿日期:2021-04-07
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  • 录用日期:2021-06-05
  • 在线发布日期: 2022-09-15
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