As a characteristic agriculture of Hebei province，facility melon cultivation has widespread problems such as continuous planting and excessive application of fertilizers and pesticides，which can cause the deterioration of soil properties and the imbalance of micro-ecological flora.Three basal fertilizers were adopted，including decomposed sheep manure，soil conditioners and bio-organic fertilizers.The effects of different base fertilizer measures on soil physical and chemical properties，bacterial diversity and melon yield were studied.The results showed that sheep manure，soil conditioner and bio-organic fertilizer increased the pH of 0～20 cm soil layer and the total number of cultivable microorganisms by 1.94% and 9.10% on average，and decreased soil bulk density，total water-soluble salt and fungi count by 6.25%，24.68% and 6.80%，respectively.Compared with bio-organic fertilizer(T1)，decomposed sheep manure(T2)，bio-organic fertilizer+soil conditioner(T3)and decomposed sheep manure+soil conditioner(T4)treatments increased the abundance of Proteobacteria，Acidobacteria，Nitrospirae，etc.，and decreased the abundance of Chloroflexi，Bacteroidetes，etc.，T3 and T4 significantly increased the abundance of soil bacteria.Compared with T1，T4 significantly increased the available phosphorus，available potassium，microbial count，bacteria count，and bacteria/fungus of the cultivated layer by 29.07%，57.65%，20.82%，21.07% and 11.93%，respectively，reduced soil bulk density by 6.68%，and increased yield by 2.87%. By comprehensive comparison，decomposed sheep manure+conditioner can not only significantly improve the soil micro-ecological environment，but also increase yield and quality，and can be recommended for application in facility muskmelon cultivation in Hebei province.