不同耕作方式下玉米秸秆还田对土壤真菌群落的影响
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(1.宁夏大学农学院,宁夏 银川 750021;2.灵武市农业技术推广中心,宁夏 灵武 750400;3.宁夏农林科学院农作物研究所,宁夏 银川 750002)

作者简介:

吕开源(1997-),硕士研究生,主要从事玉米秸秆还田影响机理研究。E-mail:913637685@qq.com。

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基金项目:宁夏回族自治区农业高质量发展与生态保护科技创新示范课题(NGSB-2021-3-02);宁夏回族自治区重点研发计划项目(2019BBF02003,2018BBF02018)。


Effects of maize straw returning on soil fungal community under different tillage methods
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(1.College of Agriculture of Ningxia University,Yinchuan Ningxia 750021;2.Lingwu Agricultural Technology Extension and Service Center,Lingwu Ningxia 750400;3.Crop Research Institute of Ningxia Academy of Agriculture and Forestry,Yinchuan Ningxia 750002)

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    摘要:

    研究宁夏引黄灌区秸秆还田与籽粒直收玉米配套模式下不同耕作和秸秆还田方式对土壤真菌群落组成及功能的影响,探究土壤真菌群落对耕作和秸秆还田方式响应差异的生物学机制,为优化耕作与秸秆还田方式和提高农田土壤肥力提供理论依据。以宁夏引黄灌区秸秆还田与籽粒直收玉米连作农田土壤为研究对象,采用Illumina MiSeq高通量测序技术,分析2种耕作方式(免耕与深翻)与3种秸秆还田方式(不还田、秸秆半量还田与秸秆全量还田)定位试验条件下,玉米成熟期土壤真菌群落结构与功能的差异,并结合土壤理化性质,进一步探究农田土壤真菌群落结构及功能变化的环境驱动因子。结果表明,免耕结合秸秆半量覆盖还田处理土壤真菌多样性指数表现最优,免耕条件下秸秆不还田与秸秆半量还田处理土壤真菌OTU数均多于深翻,秸秆全量还田处理则相反。各处理优势真菌种群存在明显差异,子囊菌门、担子菌门、被孢菌门、粪壳菌纲、Symmetrospora属、被孢霉属和子囊菌属在各处理中相对丰度普遍较高;免耕处理对不同真菌物种存在不同的影响,可以促进参与木质素腐解的真菌生长发育,降低致病真菌的相对丰度。耕作方式对真菌群落组成的影响显著,免耕结合秸秆不还田、秸秆半量覆盖还田处理下土壤真菌群落组成稳定性较强,其余处理的土壤真菌群落组成稳定性则较差。各处理对真菌群落组成贡献较大的真菌物种存在差异。土壤理化性质与不同菌属的生长繁殖关系密切,主要优势菌属主要受土壤碱解氮、全磷、有效磷、容重的影响。本研究发现,不同耕作与秸秆还田方式改变了农田土壤真菌群落OTU数量、多样性参数以及群落组成,不同的土壤理化性质也是影响各真菌菌属生长繁殖的主要因素;免耕结合适宜的秸秆覆盖还田量能够促进土壤中易腐解秸秆的菌属生长繁殖,利于形成秸秆还田生态圈的良性循环,促进农田土壤生态系统稳定。

    Abstract:

    Studying the effects of different tillage and straw returning methods on the composition and function of soil fungal community under the matching mode of straw returning and direct-groin corn harvesting in Ningxia Yellow River Diversion Irrigation Area,and investigating the biological mechanisms of soil fungal communities in response to different tillage and straw harvesting methods,in order to provide theoretical basis for optimizing tillage and straw harvesting methods and improving soil fertility.Taking the continuous cropping soil of straw returning to field and direct-grain corn harvesting in the Ningxia Yellow River Diversion Irrigation Area as the research object,Illumia Miseq high throughput sequencing technology was used to analyze the differences in soil fungal community structure and function during the maize maturity period under the conditions of two farming methods(no-tillage and deep-tillage)and three straw returning methods(no-straw returningto the field,returning half amount of the straw to the field,and returning full amount of straw to the field),and combined with the soil physical and chemical properties,and the environmental drivers of soil fungal community structure and function changes in farmland were further investigated.The results showed that the soil fungal diversity indices were best in no-tillage combined with half amount of straw mulching,and the number of soil fungal OTU was higher in both no-tillage and half amount of straw mulching treatments than in deep-tillage,while the opposite was true for full amount of straw mulching treatment.The dominant fungal populations were significantly different among treatments,with Ascomycota,Basidiomycota,Mortierellomycota,Sordariomycetes,Symmetrospora,Mortierella and Archaeorhizomyces generally having higher relative abundance in each treatment.No-tillage treatment had different effects on different fungal species,and it promoted the growth and development of fungi involved in lignin decay and reduced the relative abundance of pathogenic fungi.No-tillage combined with no-straw return and straw half mulch return treatments were more stable,while the remaining treatments were less stable.The fungal species that contributed more to the fungal community composition differed among treatments.Each soil physicochemical property was closely related to the growth and reproduction of different fungal genera,and the main dominant fungal genera were mainly influenced by soil alkaline nitrogen,total phosphorus,available phosphorus and bulk density.Different tillage and straw return methods change the number of OTU,diversity parameters and community composition of soil fungal communities,and different soil physicochemical properties are also the main factors affecting the growth and reproduction of each fungal genus;no-tillage combined with appropriate amount of straw mulch return can promote the growth and reproduction of fungal genera in soil that can easily decompose straw,which is conducive to the formation of a virtuous cycle of straw return ecosphere and maintain the health of farmland soil ecosystem.

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吕开源,周立萍,康建宏,吴宏亮,贾彪,班文慧,蔡启明.不同耕作方式下玉米秸秆还田对土壤真菌群落的影响[J].中国土壤与肥料,2022,(8):112-122.

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  • 收稿日期:2021-05-26
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  • 录用日期:2021-09-11
  • 在线发布日期: 2022-10-10
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