In order to clarify the regulation effect and mechanism of slow-released fertilizer on the yield and quality of strong gluten wheat，and to explore the scientific and reasonable application technology of slow-released fertilizer on strong gluten wheat.Taking strong gluten wheat variety “Nongmai 88” as material，using sulfur coated slow-release fertilizer(SCU，N-P2O5-K2O=26-12-12)and urea(U，N 46.3%)as nitrogen fertilizers，six fertilization patterns were designed:U applied at four stages as the control(CK)；100%N-SCU applied before sowing(M1)；60%N-SCU applied before sowing，40%N-U applied at jointing stage(M2)；60%N-SCU applied before sowing，40%N-SCU applied at re-greening stage (M3)；15% total nitrogen reduction based on M2(M4)；15% total nitrogen reduction based on M3(M5).The differences of nitrogen accumulation and translocation，nitrogen efficiency，yield and quality of strong gluten wheat under different fertilization patterns were analyzed.The results showed that，compared with CK，post-anthesis nitrogen remobilization amount and nitrogen remobilization rate of M3 showed a decreasing trend，but the difference was not significant.The post-anthesis nitrogen accumulation and its contribution to grain were significantly increased in M3 compared with those in CK，which contributed to the significant increase in shoot and grain nitrogen accumulation amount at maturity stage，and increased nitrogen apparent utilization rate and agronomic efficiency by 8.56% and 10.72%，respectively.The rational nitrogen supply in M3 promoted the increase of spikes and 1000-grain weight，and the yield increased by 7.01% compared with CK.The grain protein content，wet gluten content，dough formation time，stabilization time and maximum tensile resistance in CK and M3 showed no significant difference，but were significantly increased compared with those in M1.Compared with M3，M1 significantly decreased grain nitrogen accumulation，nitrogen apparent utilization rate and grain yield，and was not conducive to the quality formation of strong gluten wheat.Under the condition of 15% nitrogen reduction，M5 significantly improved nitrogen apparent utilization rate and increased grain yield by 2.44% compared with CK.M5 significantly decreased the grain protein content，wet gluten content，dough formation time and stability time compared with CK，but showed certain advantages in quality indexes compared with M1.In this study，different application patterns of sulfur coated slow-released fertilizer have strong regulatory effects on nitrogen uptake and utilization，yield and quality of wheat.The pattern of 60%N-SCU applied before sowing with 40%N-SCU applied at re-greening stage promoted the synergistic increase in grain yield，nitrogen efficiency and quality of strong gluten wheat，and showed potential of reducing nitrogen with efficiency increasing，which is a high-yield，high-efficiency and good-quality fertilization method.