The principal.component.analysis and the comprehensive score were used to evaluate the fertilizer effect of substitution of chemical fertilizer by different kinds of organic fertilizers，to clarify the effect of different kinds of organic fertilizer substitution on the growth and development of Ponkan citrus，yield and quality，for the scientific reduction of citrus fertilizer，the rational application of organic fertilizer and its quality and efficiency to provide theoretical basis and technical support.Field tests were carried out with 15-year-old Taiwan Ponkan grafted on Poncirus trifoliata(L.)as test materials，and six treatments were set up，including chemical fertilizer alone(CK)，commodity organic fertilizer substitution(T1)，bio-organic fertilizer substitution(T2)，smoke stem bio-organic fertilizer substitution(T3)，composite microbial organic fertilizer substitution(T4)and oil cake organic fertilizer substitution(T5)，and the effects of different treatments on the growth and development，physiology and yield，quality of Ponkan citrus were analyzed.The results showed that the growth of branches and roots treated by organic fertilizer was better than CK in general，with T3 and T4 being the best，of which the total length of roots and the number of roots treated by T3 were 24.8% and 35.8% higher than CK，respectively，and the improving effect of distribution ratio of root system with T2 and T3 treatments was better.The fruit dry matter of organic fertilizer substitution treatments increased by 20.2% to 25.9% compared with CK，with T2 treatment as the largest. The root vigor of Ponkan citrus was the highest in May and smaller in September.The root vigor of Ponkan citrus treated with T2 and T3 was stronger than other treatment.The nitrogen，phosphorus and potassium contents of Ponkan citrus leaves，fruit of T3 and T4 were relatively high.The single fruit weight treated with T3 was the highest in 2019 and 2020. The yield of each organic fertilizer treatment was increased by 19.4% to 26.9% and 23.1% to 34.0%，respectively，compared with CK，of which was relatively high in T3 and T2 treatments，respectively.The appearance quality of fruit was improved，and the treatments of T2 and T3 was relatively better；the intrinsic quality of the fruit treated with organic fertilizer was improved compared with CK，and the fruit treated with T2 and T3 was better overall.The comprehensive score order of the principal.component.analysis of each treatment was T3> T2> T5> T1> T4 > CK，that is，the fertilizer effect of each organic fertilizer treatment was better than CK，especially with T3 treatment，which was relatively optimal.