Through the field experiment with selenium-rich soil(soil selenium content was about 1.0 mg/kg)，different concentrations(CK:0 g/hm²，T1:20 g/hm²，T2:40 g/hm²，T3:60 g/hm²，T4:80 g/hm²，T5:100 g/hm²，T6:120 g/hm²) of organic selenium-enriched liquid fertilizer(organic selenium≥6.0 g/L)were sprayed on rice leaves at grain filling stage(6 and 12 d after anthesis)to explore the selenium content and distribution characteristics of aboveground parts，and analyze the changes of yield and quality.The results showed that selenium fertilizer spraying had no significant effect on plant height，panicle length，branch number，effective panicle number and spikelets per panicle(P>0.05)，but it had obvious promotion effect on seed setting rate，1000-grain weight and actual yield.The promotion effect was weakened when the concentration was too high(120 g/hm²)，and the correlation between selenium fertilizer concentration and yield reached a significant level(R=0.7850，P<0.05).With the increase of spraying concentration of selenium fertilizer，the selenium content in different tissues all significantly linearly increased(P<0.01)，and the correlation coefficient was more than 0.9.Although the 0.32 mg/kg selenium content of rice of CK had exceeded the standard of selenium-rich rice(0.04～0.30 mg/kg)，it could ensure that the daily selenium intake of rice was within the appropriate range of daily dietary selenium supply(50～250 μg/d，based on 400 g rice per day).When the spraying concentration was 120 g/hm²，the daily selenium intake of rice was 276 μg(based on 400 g rice per day)，which exceeded the suitable range of daily dietary selenium supply.Selenium spraying increased the proportion of selenium accumulation in stem sheath，leaf and cob+branch，while the proportion of selenium content in milled rice，rice bran and rice husk decreased.Different spraying concentrations of selenium fertilizer significantly reduced the chalky grain rate(P<0.05)，but increased the head rice rate and chalkiness(P<0.05)，and had no significant effect on brown rice rate，milled rice rate and grain length-to-width ratio(P>0.05).At the same time，the application of selenium fertilizer decreased the protein content of rice and made the gel consistency longer，while the amylose content and alkali spreading value had no significant change(P>0.05).In summary，the application concentration of 100 g/hm² selenium fertilizer could significantly promote the accumulationof dry matter at maturity，increase the chlorophyll content of flag leaf，and delay the senescence of flag leaf and increase the harvest index to promote the increase of yield；it could increase the selenium content in aboveground parts of plant to ensure that the daily selenium intake of rice was in the appropriate range，and improve the processing quality，appearance quality and cooking and eating quality of rice.