The aim was to provide excellent bacteria resources for microbial fertilizers suitable for rice production in the middle and lower reaches of the Yangtze River. Six PGPR strains A2，A5，A8，A11，A16 and A18 were isolated and screened from the rhizosphere of Dongxiang wild rice by the selective medium method，and the growth-promoting characteristics and ecological adaptability of the strains were determined. The results showed that the six PGPR strains isolated and screened differed in their growth-promoting characteristics and ecological adaptability. Strains A5 and A18 were able to dissolve phosphorus，strains A2，A5，A11 and A18 were able to produce siderophores，strains A5，A8 and A18 were able to dissolve potassium，and all 6 strains were able to produce IAA and ACC deaminase；strains A5 and A18 exhibited strong acid and alkali resistance，and grew vigorously under different acid and alkali conditions；strains A5 and A18 could grow on a medium with a NaCl concentration of 1.2 mol·L-1，showing extremely strong salt tolerance；strains A5，A8 and A18 could grow on a medium with a polyethylene glycol content of 30%，indicating that they could tolerate severe drought；strains A2，A5 and A18 grew on media containing different dilutions of acetochlor，indicating that they could tolerate herbicides. The 6 strains could grow on the culture medium containing different concentrations of azoxystrobin and imidacloprid. Among them，the strains A5 and A18 grew most vigorously，indicating that all the 6 strains were all resistant，and the strains A5 and A18 were the most tolerant. Preferably，the strains A5 and A18 were analyzed and identified by the 16S rDNA gene sequence. The strain A5 was Pseudomonas vancouverensis，and the strain A18 was Pantoea cypripedii. The rice field test results showed that with 50% reduction of nitrogen fertilizer，the yield of rice inoculated strains A5，A18 and A5+A18 increased by 23.11%，17.33% and 29.68%，respectively，which indicated that the inoculation of strains A5，A18 and A5+A18 had a very good effect on the growth and production of rice，and the effect of inoculation with compound bacteria was the best.