The aim is to explore the fertilizer application rates to obtain high yield，high quality，and high nutrient use efficiency of spring wheat in Hetao irrigation district.A four-level completely random block test of phosphorus and nitrogen were set up based on farmer habitual fertilization amount of P2O5 241.9 kg·hm-2，N 370.5 kg·hm-2 in field under the condition of applying 225 kg·hm-2 bio-organic fertilizer.The effects of phosphorus and nitrogen application on grain yield，quality and plant nutrient use efficiency under base application of bio-organic fertilizer were determined.With the decrease of nitrogen application rate and phosphorus application rate，the yield，spikes，grains per spike and 1000-grain weight all showed the trend of increasing first and then decreased.Under the same nitrogen level，the plant nitrogen content，nitrogen accumulation，nitrogen recovery efficiency，nitrogen agronomic efficiency，nitrogen partial factor productivity and plant phosphate content，phosphate accumulation，phosphate agronomic efficiency showed a single peak curve with the decrease of phosphorus application rate，while the phosphate recovery efficiency，phosphate partial factor productivity showed a gradually increasing trend.Under the same level of phosphate，the various nitrogen and phosphorus fertilizer use efficiency indicators showed a single-peak curve change trend with the decrease of nitrogen application rate.Under the same nitrogen level，grain quality indicators showed a trend of first increasing and then decreasing with the decrease of phosphorus application rate.Under the same phosphorus application，except for water absorption gradual decrease，other quality indicators showed a trend of first increasing and then decreasing with the decrease of nitrogen application rate. The recommended phosphorus(P2O5)and nitrogen application rate (pure N)for high-yield，high-quality and high-use efficiency is 158.23～166.07 and 274.93～287.36 kg·hm-2，respectively，combined with 225 kg·hm-2 biological organic fertilizer.Under this fertilization mode，the amount of phosphorus can be reduced by 31.35%～34.59%，and the amount of nitrogen can be reduced by 22.42%～25.79%.