The Huang-Huai-Hai plain is an important major grain producing area in China.In this area，the utilization efficiency of nitrogen fertilizer is low and therefore a large amount of fertilizer is lost.To provide useful information for overcoming this problem，in this study，the National Soil Quality Observation and Experimental Station in Xinxiang was employed as our research platform to monitor the soil of a wheat-corn rotation system under long-term fertilization in different modes.Specifically，the nitrogen loss due to volatilization was monitored，with the purpose of exploring scientific fertilization strategies to reduce the soil ammonia volatilization in the Huang-Huai-Hai plain，and to provide knowledge for improving the utilization efficiency of nitrogen fertilizer.This study contained five treatments:control (CK)，single application of chemical fertilizer (NPK)，single application of organic fertilizer (M)，straw materials that were returned to field combined with chemical fertilizer (NPK+S)，and chemical fertilizer plus organic fertilizer (NPK+M).The loss of soil ammonia due to volatilization was observed in the wheat growing season，and the effects of soil mineral nitrogen content and soil pH on soil ammonia volatilization were analysed.The results showed that long-term fertilization had a significant effect on wheat yield and nitrogen absorption.The yield of wheat in each fertilization treatment was significantly higher than that of CK treatment，and the yields of grain，straw and glume were the highest in NPK+M treatment，reaching 11.6 t·hm-2(a 15.6% increase compared to the value of the NPK treatment)，13.38 t·hm-2 (39.1%)and 3.34 t·hm-2 (18.4%)，respectively.The NPK+M treatment resulted in the largest nitrogen uptake by wheat shoots in all treatments，reaching 306.67 kg·hm-2，which was 14.3% and 44.7% higher than that of NPK and NPK+S treatments，respectively.Different fertilization treatments had a significant impact on soil ammonia volatilization.The peak of soil ammonia volatilization rate in the wheat growing season occurred mainly within 1～4 d after fertilization，and NPK+S treatment induced the highest peak value，reaching N 0.40 kg·hm-2·d-1，followed by NPK+M treatment.In the wheat growing season，the total amount of soil ammonia loss due to volatilization under different long-term fertilization treatments reached 9.73～13.73 kg·hm-2，and the total amount of ammonia volatilization in the basal fertilizer period (52.7%～64.3% of the fertilization amount)was higher than that in the top-dressing period.The NPK+S treatment resulted in the highest level of soil ammonia volatilization in the wheat growing season (13.73 kg·hm-2)，which was significantly increased by 24.5% compared with that of the NPK treatment.However，the total amount of ammonia volatilization from the soil in the wheat growing season under the NPK+M treatment only accounted for 4.65% of nitrogen application，which was significantly lower than other fertilization treatments.In summary，the combined application of organic and inorganic fertilizers can significantly increase wheat yield and nitrogen fertilizer absorption，and reduce soil ammonia volatilization；thus，this strategy might be able to promote crop yields and improve the efficiency of fertilizer utilization.Based on our results，we propose this combination as a potentially optimal fertilization strategy for wheat production in the Huang-Huai-Hai plain.