To investigated the effects of the biological organic fertilizer HZ-24 on enhancement of quantity and quality of A. mongholicus’ original habitats，and provided theoretical basis for the application of biological organic fertilizer HZ-24 on A. mongholicus crop production.Four treatments were test on Astragalus mongholicus Bunge in field trials:no fertilizer (CK)；chemical fertilizer (T)；20% reduced chemical fertilizer (J)；20% reduced chemical fertilizer+biological organic fertilizer HZ-24 (HZ).High-throughput sequencing，HPLC and routine chemical methods were conducted to analyze the effects of HZ-24 on rhizosphere microbiology community and related nutrients of A. mongholicus，and the influences of the crop quality and yield.As the results showed，the yield of HZ treatment had no significant difference from T，on the other hand，the yield of HZ increased 7% compared with J.The enhancements of HZ in contrast with CK，T，J on organic mass of rhizosphere soil were 11.92%，9.04%，8.96%；the ammonium nitrogen content were 51.37%，16.52%，27.5%；the available phosphorus were 44.17%，18.92%，18.20%；and on the available potassium were 21.88%，26.32%，18.63%，respectively.The concentration of Astragaloside IV in the root of A. mongholicus were 17.81% higher in HZ treatment than in that T，and the concentration of Calycosin were 13.28% higher.The unidentified_Bacteri in the rhizosphere in HZ group were 60.53% less than CK，and the Chloroflexi was 14.88% less in HZ group than T.Dongia，Ferruginibacter，Ferruginibacter，Phenylobacterium，Bryobacter，Rhodoplanes，Lysobacter from Protebacteria were the dominant genus in HZ treatment groups.In conclusion，the rhizosphere soil bacterial community was significantly altered by the utilization of HZ-24，and the rhizosphere microecosystem and soil nutrients were also changed to better condition，thus，it improved the crop quality of A.mongholicus.