落干对表层水稻土氮素转化及关键功能微生物丰度的影响
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(1.中国科学院亚热带农业生态研究所桃源农业生态试验站,亚热带农业生态过程重点实验室,湖南 长沙 410125;2.中国科学院大学资源与环境学院,北京 100049)

作者简介:

易恋淳(1997-),硕士研究生,研究方向为土壤微生物生态。E-mail:yilianchun19@mails.ucas.ac.cn。

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基金项目:中国科学院科技服务网络项目(KFJ-STS-QYZD-165);国家重点研发计划项目(2016YFD0200307);中国科学院野外站联盟项目(KFJ-SW-YW035)。


The effects of drying process on nitrogen conversion and abundance of key functional microorganisms in surface paddy soil
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(1.Key Laboratory of Agro-ecological Processes in Subtropical Regions,Taoyuan Station of Agro-Ecology Research,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha Hunan 410125;2.College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049)

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    摘要:

    落干过程中稻田土壤氮素转化发生显著变化,但表层土壤不同层次的氮素转化特征及相关机制尚不清楚。通过室内土壤培养试验,探讨了落干过程中(9 d)上表层(0~5 cm)和下表层(5~10 cm)水稻土氧化还原电位(Eh)、土体氧化亚氮(N2O)浓度和排放通量等的动态变化特征,并利用实时荧光定量PCR测定氨氧化基因(古菌amoA和细菌amoA)和硝酸盐还原酶基因(narG和napA)的丰度变化。结果表明,经9 d落干上表层水稻土Eh由-200 mV上升至500 mV左右,而下表层在-200~0 mV之间波动。上表层水稻土NH4+-N含量下降速率和NO3--N含量上升速率分别是下表层的2.8和1.8倍。落干过程中,上表层水稻土氨氧化作用可能是由氨氧化细菌(AOB)主导的,而驱动硝酸盐还原作用可能以含napA基因反硝化微生物为主。

    Abstract:

    The nitrogen conversion in paddy soil changed significantly during the drying process,but the characteristics and related mechanisms of nitrogen transformation at different soil layers were unclear.In this experiment,the dynamic characteristics of soil redox potential (Eh),soil N2O concentration and emission flux in upper surface layer (0~5 cm)and lower surface layer (5~10 cm)during 9 days of drying were studied by soil incubation experiment.The abundance changes of ammoxidation genes (archaea amoA and bacteria amoA)and nitrate reductase genes (narG and napA)were determined by real-time quantitative PCR.The results showed that the soil Eh in the upper surface layer increased from -200 to about 500 mV after 9 days of drying,while the soil Eh in the lower surface layer fluctuated from -200 to 0 mV.The decreasing rate of NH4+-N content and increasing rate of NO3--N content in the upper layer of paddy soil were 2.8 and 1.8 times of that in the lower layer,respectively.In the drying process,ammonia-oxidizing bacteria (AOB)may be dominant in the upper surface of paddy soil,while napA-containing denitrifying microorganisms may be dominant in driving nitrate reduction.

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易恋淳,张文钊,盛荣,魏文学.落干对表层水稻土氮素转化及关键功能微生物丰度的影响[J].中国土壤与肥料,2022,(11):174-182.

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  • 收稿日期:2021-10-13
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  • 录用日期:2021-12-27
  • 在线发布日期: 2023-02-07
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