The analysis and detection method of 8 kinds of sedatives and 15 kinds of β- Receptor hormone drug residues in organic fertilizers were established by magnetic graphene solid phase extraction combined with liquid chromatography-tandem mass spectrometry.The sample was extracted with 0.1% formic acid acetonitrile.The target was adsorbed by magnetic graphene powder to remove impurities，and then the target was analyzed to obtain a clean sample matrix.Chromatographic column of Agilent ZORBAX Eclipse Plus C18 (3.0 mm×100 mm，1.8 μm)was used for separation，liquid chromatography-tandem mass spectrometry was adopted for detection，and standard curve internal standard method was used for quantification. The results showed that the linear of 23 targets was good in the range of 0.5～10.0 μg/L (R2>0.999).The average recoveries were 75.1%～102.4% in 2，5 and 10 μg/kg，and the relative standard deviation was 4.3%～14.3% (n=6).The method has high purification efficiency and wide application range，and can be used in the screening and detection of sedatives and β- receptor hormone drug residues in organic fertilizers.