To focused on optimal nitrogen(N)fertilizer application rate and strategy on the main and ratoon crop of ratoon rice，so as to provide references for high yield，excellent quality and high utilization of N fertilizer.Hybrid rice of Fengliangyouxiang no.1 was tested by a field experiment in Jiangling county，Hubei province，which contained 7 treatments:no fertilizer(CK)，no N fertilizer(N0)，conventional N fertilizer application rate(280 kg/hm²)and conventional N fertilizer strategy (Nc)，conventional N fertilizer application rate and increased seedling fertilizer ratio(NCC)，conventional N fertilizer application rate and 100% seedling fertilizer(Ntz)，N reduction 20% and increased seedling fertilizer ratio(N-cc)，N reduction 20% and 100% seedling fertilizer(N-tz).Results showed that，at the same N strategy and compared with no N reduction treatment，N reduction 20% treatment produced basically equal yield，a little bit lower N uptake，relatively higher N use efficiencies(NUEs)，and significantly higher partial factor productivity of applied N(PEPN).While compared with Nc treatment，Ntz and N-tz treatments increased the total grain yield by 9.8% ～ 11.2%，the head milled rice rate increased by 3.5 ～ 4.2 percentage，chalkiness degree decreased by 0.7 ～ 1.4 percentage，N harvest index increased by 9.8% ～ 14.8%，N accumulation for 100 kg seeds decreased by 3.0% ～ 10.8%，N contribution rate increased by 7.6 ～ 8.5 percentage，PEPN increased by 11.2% ～ 36.7%，N agronomic efficiency increased by 74.0% ～ 105%，N recovery efficiency increased by 4.3 ～ 6.6 percentage，N physiological efficiency increased by 34.3% ～ 70.8%.Additionally，most NUEs of the ratoon rice were higher than those of the main rice.Therefore，the optimal N application rate was around 225 kg/hm²，65% of which be applied in the first rice，and 35% in the ratoon rice.On the basis of applying sufficient panicle fertilizer in the first rice，N fertilizer in the ratoon rice can be used as seeding fertilizer.