A phosphate-solubilizing bacterial strain was isolated from the rhizosphere soil of rice and other crops using the traditional method.This isolated strain were able to use Ca3(PO4)2 as sole source of phosphorus.It was identified as Bacillus megaterium sp.according to its morphology and biochemical properties analysis.The optimal pH value and temperature for its growth in inorganic phosphorus medium were 7 and 35 ℃，respectively.The phosphate-solubilizing capacity of the isolated strain was confirmed by the phosphate-solubilizing zone and the increase in soluble phosphorus in liquid cultures.In addition，the isolates also promoted the growth of Escherichia coli on solid medium.The isolated phosphate-solubilizing bacterial strain has great potential in further development of microbiological fertilizer because of its phosphorus dissolving ability and growth promoting effect.