The seedling-box total fertilization technology is a simple and efficient controlled-release urea(CRU)fertilization technology，which is one-time application of CRU near rice seeds during the rice seedling raising，and the fertilizer is attached to the rice root system and brought into the paddy field when transplanting.In order to study the effects of different application rates and raising seedlings methods of CRU on rice seedling growth，the local conventional nitrogen application rates (180 kg/hm²，1550 g CRU was used per seedling tray)was used as the benchmark，five CRU rates (CK，25% CRU，50% CRU，75% CRU and 100% CRU)were set up in this study，for the 50% CRU treatment，three including seedling ways were studied，including boxing-tanzhuang(in Chinese)seedling trays(50%CRU+B)，the soil-fertilizer-seeds filling methods(50%CRU +S)，and the combined seedling ways of B and S(50%CRU +BS).The results showed that CRU fertilization rates by coexistence placement should not exceed 745 g per seedling tray.The seedling quality of 50%CRU treatment was the best，while 75%CRU and 100%CRU treatments had different inhibitory effects on seedling growth.Compared with conventional seedling raising(CK)，50% CRU had similar emergence rate，uniformity，seedling strengthening index and plumpness，but significantly increased the root dry weight，shoot dry weight and root activity by 52.25%，73.22% and 21.23%，respectively.With the increase of the amount of controlled-release urea，the water content of seedling soil decreased，and the NH4+-N and electrical conductivity of soil increased.Compared with CK，the adverse growth environment caused by 75% CRU and 100% CRU increased MDA of seedling by 46.6%～272.0%，and decreased SOD and CAT content by 22.8%～30.5% and 6.5%～29.2%，respectively.The three optimized seedling raising methods can improve the seedling height and seedling strengthening index，but had no significant effect on other seedling growth indicators.In conclusion，the seedling growth was the best when CRU was used as 745 g per seedling tray(about 90 kg/hm²)as contact-application way.The optimized seedling raising method promoted the seedling strengthening index and seedling height，but had no significant effect on other growth indexes of seedlings.