Selenium is easy to be fixed in the acidic soil in the south and its effectiveness is low，which affects the absorption of selenium in tea trees in this region.The effects of phosphorus，amino acids and fulvic acid on soil selenium availability in southern tea plantation soil were studied，so as to provide a theoretical basis for improving soil selenium availability and tea selenium uptake in southern selenium-rich tea plantation.A field experiment was conducted with three factors，including phosphorus(P)，amino acid(A) and fulvic acid(H).There was two phosphorus levels，including (168.8 kg/hm² P1) and (337.5 kg/hm² P2)，and there was no gradient for amino acid or fulvic acid，which was 112.5 kg/hm².A total of 7 treatments were set up，including CK，A，P1-A，P2-A，H，P1-H and P2-H.The effects of organic materials alone or combined application of phosphorus and organic materials on selenium content in tea and selenium availability in tea plantation soil were studied.The results showed as follows:(1)The selenium content of tea with single application of amino acid(A)and combined application of amino acid and high phosphorus(P2-A) was significantly higher than CK，which was increased by 40.03% and 20.40%，respectively.(2)Single application of amino acid(A)and combined application of amino acid and high phosphorus(P2-A)significantly increased the soil available selenium content.The increase of available selenium by applying amino acid(A)was mainly due to the increase of soluble selenium，while combined application of amino acid and high phosphorus(P2-A)mainly came from the increase of soluble selenium and exchangeable selenium.(3)Both single application of amino acid(A)and combined application of amino acid and high phosphorus(P2-A)increased the content of tetravalent selenium in soluble selenium，which was the main reason for the increase of selenium content in tea.Amino acids and fulvic acids are organic acids，but amino acids in the tea plantations of latosolic red soils in southern China have better effects on soil selenium，and application of amino acids alone or in combination with amino acid and phosphorus can improve soil selenium availability and then improve selenium absorption in tea leaves.