The aimed to find out the optimal irrigation amount and fertilization amount of substrate culture tomato with corn straw and cow dung as main raw materials，and provide scientific basis for irrigation and fertilization system of tomato organic substrate culture.The decomposed corn straw，decomposed cow dung and river sand，vermiculite，perlite，attapulgite and other materials were compounded into cultivation matrix according to a certain proportion.The effects of different water and fertilizer conditions on the growth，yield，water and fertilizer utilization efficiency of tomato cultivated in matrix in solar greenhouse were studied，and the production cost and economic benefit of tomato under different water and fertilizer conditions were analyzed.The results showed that different irrigation amount and fertilizer amount had great influence on the growth and yield of tomato cultivated in organic matrix.Under the same irrigation amount，the crop yield increased first and then decreased with the increase of fertilization amount，and the tomato yield increased with the increase of irrigation amount under the same fertilization amount.When the maximum irrigation amount and medium fertilization amount were combined together，the tomato yield was the highest 3.73 kg/plant，which was 10.9%～83.0% higher than that of other treatments；the highest water use efficiency was 18.45 kg/m3，which was 10.8%～50.1% higher than other treatments；the production income was the best，which was 10.43 yuan/plant，and increased by 10.9%～87.2% compared with other treatments；the net income was 6.00 yuan/plant，which was 17.7%～363.2% higher than other treatments.When the irrigation amount was 162.00 L/plant and the fertilization amount was 41.53 g/plant in the whole growth period of tomato，the growth indexes such as plant height，stem diameter，leaf number and SPAD value of tomato were the best.From the comprehensive analysis of production factors such as yield increasing，water use efficiency and economic benefit，this irrigation amount and fertilization amount were the best combination for the organic-inorganic ecological substrate cultivated tomato with corn straw and cow dung as the main raw materials.