Phosphorus application to infertile agricultural soils has greatly contributed to achieving increased crop yield and food production.However，the excessive and long-term use of phosphates negatively impacts the environment and causes crop yield decreasing.Using alternative and sustainable approaches including phosphorus-solubilizing microorganisms are imperative.As such，this study aimed to screen phosphorus-soluble strains that can adapt to the cold climatic environment of the Qinghai-Tibet Plateau.PaenibBacillus mucilaginosusv，Priestia megaterium，Bacillus cereus，Streptomyces violovariabilis，Streptomyces cinnamofuscus，and Streptomyces flavoagglomeratus were inoculated in low phosphorus soil(available phosphorus:1.48 mg/kg，total phosphorus:1.3 g /kg)，and experimental soils were incubated at 5，15 and 25 ℃ to simulate characteristic temperatures of crop growth in the cold environment of the Qinghai-Tibet Plateau.Soil pH，available phosphorus content，alkaline phosphatase activity，and inorganic phosphorus composition at different temperatures were analyzed，and the phosphorus-solubilizing bacteria with strong phosphorus-solubilizing ability were screened.The results showed that the available phosphorus content and alkaline phosphatase activity of soil inoculated with S.violovariabilis were higher than those of other inoculated strains at any temperature，and there was a significant negative correlation between pH and available phosphorus content of soil.The contents of Ca2-P and Ca8-P in inorganic phosphorus at 15 ℃ were higher than those of other treatments.Therefore，S.violovariabilis is a phosphorus solubilizing strain suitable for the cold climate environment in Qinghai.