To evaluate the effects of combined application of chemical fertilizer with manure on soil aggregate stability and its organic carbon and total nitrogen contents in 0～20 cm soil layer in greenhouse，a field experiment was carried out for seven consecutive years.There were four chemical fertilizer treatments(N0PK，N1PK，N2PK and N3PK)and four combined applications of chemical fertilizer with manure(N0PKM，N1PKM，N2PKM and N3PKM).Rate of nitrogen of N0，N1，N2 and N3 were 0，187.5，375.0 and 562.5 kg·hm-2，respectively，rate of phosphorus(P2O5)and potassium (K2O)were 225.0 and 450.0 kg·hm-2，respectively，and rate of manure was 75000 kg·hm-2.Wet-sieving was applied to extract different aggregate size fractions for studying the aggregate stability，and its organic carbon and total nitrogen contents.Compared with the application of chemical fertilizer，combined application of chemical fertilizer with manure significantly increased the soil organic carbon and total nitrogen contents(P<0.05)，by 93.21%～119.13% and 106.69%～139.42%，respectively，while N2PKM had the largest increase range，but their contents of application of chemical fertilizer and combined applications of chemical fertilizer with manure were not significantly affected by nitrogen application rate.Combined application of chemical fertilizer with manure increased the proportion of macro aggregates(>0.25 mm)and decreased that of micro aggregates(<0.25 mm)，and the mean weight diameter and stability of soil aggregates for N1PKM，N2PKM and N3PKM significantly improved(P<0.05).Combined application of chemical fertilizer with manure increased the contents of organic carbon and total nitrogen of macro- and micro-aggregate size fractions，the >2 mm aggregates were the largest increase range(285.30% and 470.83%，respectively)，and then 0.25～2 mm aggregates(235.32% and 267.25%，respectively)and 0.053～0.25 mm aggregates(113.69% and 130.54%，respectively)，and <0.053 mm aggregates were the smallest(35.13% and 34.10%，respectively).The content of soil organic carbon(total nitrogen)correlated significantly with the content of organic carbon(total nitrogen)in different aggregate size fractions(P<0.05).The 0.25～2 and 0.053～0.25 mm aggregates were the main carrier of organic carbon and total nitrogen sequestration，which were the key contributor for the improvement of soil organic carbon and total nitrogen. The combined addition of chemical fertilizer with manure in the greenhouse for seven consecutive years not only promotes the formation of macro aggregates and micro aggregates and consequently improves soil aggregate stability，but also increases the organic carbon and total nitrogen contents of soil aggregates(>0.25 mm)and therefore improves the soil organic carbon(nitrogen)pool.